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[15N标记小麦蛋白给药后蛋鸡氨基酸和蛋白质代谢的动态变化。2. 粪便中氮和碱性氨基酸的15N排泄量]

[Dynamics of amino acid and protein metabolism in laying hens after the administration of 15N-labeled wheat protein. 2. 15N excretion with nitrogen and basic amino acids of the feces].

作者信息

Gruhn K, Jahreis G

机构信息

Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig.

出版信息

Arch Tierernahr. 1987 Jun;37(6):505-20. doi: 10.1080/17450398709421066.

Abstract

Over 4 days 12 colostomized laying hens received together with a commercial ration labelled wheat with a 15N excess (15N') of 14.37 atom-%. The labelling of the basic amino acids amounted to 13.58 atom-% for lysine, to 14.38 atom-% for histidine and to 13.63 atom-% 15N' for arginine. 3 animals each were butchered 12 h, 36 h, 60 h and 108 h resp. after the last application of 15N. The heavy nitrogen in the total N and in the N fraction of non-protein origin as well as in the basic amino acids in faeces was daily determined for the individual hens in the total experimental period. On average the crude protein of faeces contained 5.45 % lysine, 2.32% histidine histidine and 3.68% arginine: the protein of faeces correspondingly contained 5.43% lysine, 2.32% histidine and 4.07% arginine. The quota of TCA soluble N in the total N of faeces amounts to one third on the 3rd und 4th days of the experiment and that of 15N' to 28%. The average atom-% 15N' of the protein fraction is 3.48 atom-% 15N' and that of the non-protein N fraction of faeces 2.93 atom-% 15N'. The apparent digestibility and that of the non-protein N fraction of faeces 2.93 atom-% 15N'. The apparent digestibility of the 14N of the ration on average amounts to 82.8% and that of the wheat 15N' to 87.5%. The average quota of the basic amino acids in the protein compounds of faeces amounts to 70.9% for lysine 15N', 73.7% for histidine 15N' and 70.3% for arginine 15N'. The digestibility of the 15N labelled amino acids amounts to 80.4% for lysine, 90.8% for histidine and 90.2% for arginine.

摘要

在4天时间里,12只接受了结肠造口术的产蛋母鸡被喂食了一种标记为小麦的商业日粮,其15N过量(15N')为14.37原子%。赖氨酸的必需氨基酸标记量为13.58原子%,组氨酸为14.38原子%,精氨酸的15N'为13.63原子%。在最后一次施用15N后,分别于12小时、36小时、60小时和108小时宰杀3只动物。在整个实验期间,每天测定每只母鸡粪便中总氮、非蛋白质来源氮部分以及必需氨基酸中的重氮含量。粪便粗蛋白平均含5.45%的赖氨酸、2.32%的组氨酸和3.68%的精氨酸;粪便蛋白质相应含5.43%的赖氨酸、2.32%的组氨酸和4.07%的精氨酸。实验第3天和第4天,粪便总氮中三氯乙酸可溶性氮的比例为三分之一,15N'的比例为28%。粪便蛋白质部分的平均15N'原子%为3.48原子%,非蛋白质氮部分为2.93原子%。粪便非蛋白质氮部分的表观消化率为2.93原子%的15N'。日粮中14N的平均表观消化率为82.8%,小麦15N'的表观消化率为87.5%。粪便蛋白质化合物中必需氨基酸的平均比例,赖氨酸15N'为70.9%,组氨酸15N'为73.7%,精氨酸15N'为70.3%。15N标记氨基酸的消化率,赖氨酸为80.4%,组氨酸为90.8%,精氨酸为90.2%。

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