Dauphinée M J, Talal N
J Immunol. 1979 Mar;122(3):936-41.
Mouse bone marrow contains theta-negative lymphocytes that can suppress an in vitro plaque response by spleen cells primed in vivo with burro red blood cells (BRBC). These bone marrow cells are radiosensitive and can be induced with thymosin fraction 5 or alpha 1 thymic peptides to express the theta antigen. Enrichment for these suppressor pre-T lymphocytes can be achieved by a one-step density centrifugation, macrophage depletion, or a combination of both procedures. NZB mice, which spontaneously develop an autoimmune disorder, have a suppressor abnormality revealed by this assay system. Upon analysis, they have normal BM pre-T suppressor cells but their spleen cells are refractory to the BM suppressor signal. NZB BM suppressor cells inhibit the response by DBA/2 spleen cells, but DBA/2 BM suppressor cells do not inhibit NZB spleen. This resistance to suppression is a property of the B cell fraction recovered from NZB spleen.
小鼠骨髓中含有θ阴性淋巴细胞，这些细胞能够抑制经体内用驴红细胞（BRBC）致敏的脾细胞的体外空斑反应。这些骨髓细胞对辐射敏感，可被胸腺素5组分或α1胸腺肽诱导表达θ抗原。通过一步密度离心、去除巨噬细胞或两种方法结合，可实现对这些抑制性前T淋巴细胞的富集。自发发生自身免疫性疾病的NZB小鼠，在此检测系统中显示出抑制异常。经分析，它们具有正常的骨髓前T抑制细胞，但脾细胞对骨髓抑制信号不敏感。NZB骨髓抑制细胞可抑制DBA/2脾细胞的反应，但DBA/2骨髓抑制细胞不能抑制NZB脾细胞的反应。这种对抑制的抗性是从NZB脾中回收的B细胞组分的特性。
