Steinmetz A, Czekelius P, Thiemann E, Motzny S, Kaffarnik H
Abteilung Endokrinologie und Stoffwechsel, Philipps-Universität, Marburg, F.R.G.
Atherosclerosis. 1988 Jan;69(1):21-7. doi: 10.1016/0021-9150(88)90285-7.
The levels, isoforms and distribution of apolipoprotein A-IV (apo A-IV) were investigated in 127 term human umbilical cord sera. In addition, apo A-IV levels and isoforms were determined on the 3rd (n = 82) and 6th (n = 68) day following parturition and compared to apo A-I concentrations. Levels of apo A-IV were low in umbilical cord serum (5.7 +/- 1.9 mg/dl) as compared to adult serum (17.6 +/- 4.8 mg/dl). No difference was found between male and female neonates. The serum distribution of apo A-IV closely resembled that seen in the adult human. Apo A-IV concentrations dramatically increased during the first week of life reaching levels of 13.4 +/- 4.1 mg/dl on day 3 and 16.7 +/- 3.4 mg/dl on day 6 post-partum. During this time apo A-I levels did not change significantly (81.0 +/- 16.5 mg/dl in cord serum, 75.3 +/- 10.6 mg/dl and 84.2 +/- 14.5 mg/dl on day 3 and 6, respectively). Cord serum already exhibited the major serum apo A-IV isoforms seen in the adult. Isofocusing of apo A-IV also identified the known genetic polymorphism of apo A-IV. Among 127 cord sera studied we identified 109 homozygote normal patterns, apo A-IV (1-1), 16 heterozygotes, apo A-IV (1-2) and 2 individuals homozygote for the variant peptide, apo A-IV (2-2). We provide evidence that apo A-IV and apo A-I are differently induced in the human neonate during the beginning of the feeding period.(ABSTRACT TRUNCATED AT 250 WORDS)
对127份足月人脐带血清中的载脂蛋白A-IV（apo A-IV）水平、异构体及分布情况进行了研究。此外，还测定了产后第3天（n = 82）和第6天（n = 68）的apo A-IV水平及异构体，并与apo A-I浓度进行比较。与成人血清（17.6±4.8mg/dl）相比，脐带血清中apo A-IV水平较低（5.7±1.9mg/dl）。男、女新生儿之间未发现差异。apo A-IV的血清分布与成年人相似。apo A-IV浓度在出生后第一周急剧增加，产后第3天达到13.4±4.1mg/dl，第6天达到16.7±3.4mg/dl。在此期间，apo A-I水平无显著变化（脐带血清中为81.0±16.5mg/dl，第3天和第6天分别为75.3±10.6mg/dl和84.2±14.5mg/dl）。脐带血清中已呈现出成年人血清中主要的apo A-IV异构体。apo A-IV的等聚焦分析还确定了apo A-IV已知的基因多态性。在研究的127份脐带血清中，我们鉴定出109例纯合子正常模式，即apo A-IV（1-1），16例杂合子，即apo A-IV（1-2），以及2例变异肽纯合子个体，即apo A-IV（2-2）。我们提供的证据表明，在新生儿喂养期开始时，apo A-IV和apo A-I的诱导情况不同。（摘要截取自250字）
