Steinmetz A, Sefraoui M, Scheffler E, Czekelius P, Barkia A, Bard J M, Parra H J, Fruchart J C, Kaffarnik H
Zentrum Innere Medizin, Institut Pasteur de Lille, France.
Gynecol Obstet Invest. 1989;28(3):127-31. doi: 10.1159/000293547.
Apolipoproteins were determined in 50 human amniotic fluids obtained by amniocentesis during weeks 16 and 22 (n = 26) or 33 and 41 (n = 24) of gestation. Whereas apo A-I, A-II, A-IV, and E were identified at levels of 1, 0.7, 0.8 and 1% of normal human adult plasma, respectively, apo B levels were only 0.04% of plasma concentration and apo C-III levels were below the detection limits of the assay. Amniotic fluid levels of apo A-II, A-IV and E did not differ between early and late stages of pregnancy, but levels of apo B decreased and apo A-I increased significantly in late pregnancy. Isofocusing showed apo A-I, A-II and A-IV in identical positions as compared to human adult plasma. Furthermore the known genetic polymorphism of apo A-IV was detectable. Individuals heterozygous, for the variant form apo A-IV-2, showing the phenotype apo A-IV (2-1), had significantly higher levels of apo A-I and A-II as compared to the common phenotype apo A-IV (1-1). We conclude that human amniotic fluid contains the major plasma apolipoproteins at about 1% of plasma levels with the exception of apo B which shows a level at an order of magnitude less than high-density lipoprotein apoproteins in comparison to their plasma counterparts.
对50份通过羊膜穿刺术获取的人羊水样本中的载脂蛋白进行了测定，这些羊水样本取自妊娠16至22周（n = 26）或33至41周（n = 24）。结果显示，载脂蛋白A-I、A-II、A-IV和E的含量分别为正常成人血浆水平的1%、0.7%、0.8%和1%，而载脂蛋白B的水平仅为血浆浓度的0.04%，载脂蛋白C-III的水平低于检测限。妊娠早期和晚期羊水载脂蛋白A-II、A-IV和E的水平无差异，但妊娠晚期载脂蛋白B水平降低，载脂蛋白A-I水平显著升高。等聚焦分析显示，与成人血浆相比，载脂蛋白A-I、A-II和A-IV处于相同位置。此外，还可检测到载脂蛋白A-IV已知的基因多态性。与常见表型载脂蛋白A-IV（1-1）相比，携带变异型载脂蛋白A-IV-2且表型为载脂蛋白A-IV（2-1）的杂合个体，其载脂蛋白A-I和A-II水平显著更高。我们得出结论，人羊水含有主要的血浆载脂蛋白，含量约为血浆水平的1%，但载脂蛋白B除外，与血浆中的对应物相比，其水平比高密度脂蛋白载脂蛋白低一个数量级。
