Biotoxin Metrology , National Research Council Canada , 1411 Oxford Street , Halifax , Nova Scotia B3H 3Z1 , Canada.
Aquatic and Crop Resource Development , National Research Council Canada , 1411 Oxford Street , Halifax , Nova Scotia B3H 3Z1 , Canada.
J Nat Prod. 2019 Jul 26;82(7):1945-1952. doi: 10.1021/acs.jnatprod.9b00318. Epub 2019 Jul 8.
Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis of a Namibian strain of showed the presence of a number of yessotoxins (YTXs). Principal among these were YTX (), homoYTX (), and a tentative hydroxylated analogue that did not correspond to any previously confirmed YTX structures. Culturing the strain afforded sufficient biomass for purification of the new analogue through a series of solvent partitioning and chromatographic steps, yielding ∼0.9 mg as a solid. NMR spectroscopy, ion-trap mass spectrometry, and HRMS identified the new analogue as 24-hydroxyYTX (). Purified 24-hydroxyYTX was quantitated by NMR, and its relative toxicity evaluated using two embryonic zebrafish toxicity assays. 24-HydroxyYTX demonstrated reduced toxicity compared to YTX.
液相色谱-高分辨质谱（LC-HRMS）分析表明，纳米比亚的一种 菌株中存在多种膝沟藻毒素（YTXs）。其中主要的有 YTX（）、同型 YTX（）和一种暂定的羟基类似物，与以前确认的任何 YTX 结构都不对应。该 菌株的培养提供了足够的生物量，通过一系列溶剂分配和色谱步骤对新的类似物进行纯化，得到约 0.9 毫克的固体。NMR 光谱、离子阱质谱和 HRMS 鉴定出这种新的类似物为 24-羟基 YTX（）。通过 NMR 对纯化的 24-羟基 YTX 进行定量，并使用两种胚胎斑马鱼毒性测定来评估其相对毒性。与 YTX 相比，24-羟基 YTX 的毒性降低。
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