从戴帽猕猴（Macaca radiata）中分离到的一种具有显著差异的猴 T 淋巴细胞性嗜亲病毒 1 缺失附属基因。
Absence of accessory genes in a divergent simian T-lymphotropic virus type 1 isolated from a bonnet macaque (Macaca radiata).
机构信息
Unité EPVO, Institut Pasteur, UMR 3569 CNRS, Paris, France.
Department of Virology, Biomedical Primate Research Centre, Rijswijk, the Netherlands.
出版信息
PLoS Negl Trop Dis. 2019 Jul 8;13(7):e0007521. doi: 10.1371/journal.pntd.0007521. eCollection 2019 Jul.
BACKGROUND
Primate T-lymphotropic viruses type 1 (PTLV-1) are complex retroviruses infecting both human (HTLV-1) and simian (STLV-1) hosts. They share common epidemiological, clinical and molecular features. In addition to the canonical gag, pol, env retroviral genes, PTLV-1 purportedly encodes regulatory (i.e. Tax, Rex, and HBZ) and accessory proteins (i.e. P12/8, P13, P30). The latter have been found essential for viral persistence in vivo.
METHODOLOGY/PRINCIPAL FINDINGS: We have isolated a STLV-1 virus from a bonnet macaque (Macaca radiata-Mra18C9), a monkey from India. The complete sequence was obtained and phylogenetic analyses were performed. The Mra18C9 strain is highly divergent from the known PTLV-1 strains. Intriguingly, the Mra18C9 lacks the 3 accessory open reading frames. In order to determine if the absence of accessory proteins is specific to this particular strain, a comprehensive analysis of the complete PTLV-1 genomes available in Genbank was performed and found that the lack of one or many accessory ORF is common among PTLV-1.
CONCLUSION
This study raises many questions regarding the actual nature, role and importance of accessory proteins in the PTLV-1 biology.
背景
灵长类 T 细胞嗜性病毒 1 型（PTLV-1）是一种复杂的逆转录病毒，感染人类（HTLV-1）和灵长类动物（STLV-1）宿主。它们具有共同的流行病学、临床和分子特征。除了规范的 gag、pol、env 逆转录病毒基因外，PTLV-1据称还编码调节蛋白（即 Tax、Rex 和 HBZ）和辅助蛋白（即 P12/8、P13、P30）。后者对于病毒在体内的持续存在至关重要。
方法/主要发现：我们从印度的一只冕毛猴（Macaca radiata-Mra18C9）中分离出一株 STLV-1 病毒。获得了完整的序列并进行了系统发育分析。Mra18C9 株与已知的 PTLV-1 株高度分化。有趣的是，Mra18C9 缺失了 3 个辅助开放阅读框。为了确定辅助蛋白的缺失是否仅限于该特定株，对 Genbank 中可用的完整 PTLV-1 基因组进行了全面分析，发现缺失一个或多个辅助 ORF 在 PTLV-1 中很常见。
结论
这项研究提出了许多关于辅助蛋白在 PTLV-1 生物学中的实际性质、作用和重要性的问题。
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