Department of Nutrition and Food Hygiene, College of Public Health of Zhengzhou University, Zhengzhou, 450001, China.
Department of Parasitology, Medical College of Zhengzhou University, Zhengzhou, 450052, China.
Eur J Pharmacol. 2019 Sep 15;859:172526. doi: 10.1016/j.ejphar.2019.172526. Epub 2019 Jul 5.
Pterostilbene (PTE) is a natural dimethylated analog of resveratrol, which exerts antioxidative, hypolipidemic and hypoglycemic effects; however, the underlying mechanism is not yet clear. In this study, we evaluated the effects of PTE on diabetic rats and clarified the underlying mechanism. Diabetes was induced in rats by streptozotocin (STZ) and a high-sugar and high-fat diet. Rats were then treated with PTE (20, 40 and 80 mg/kg/d) for 8 weeks. Oral glucose tolerance test (OGTT) was performed to measure the glycometabolism of the diabetic rats at the end of the treatment. Fasting blood glucose (FBG), fasting insulin (FINS) and lipid profile were determined using an automatic biochemistry analyzer and serum inflammatory factors were analyzed by enzyme-linked immunosorbent assay. Serum superoxide dismutase (SOD) and malondialdehyde (MDA) were also analyzed by spectrophotometry to evaluate the anti-oxidant effects. The expression of proteins of PPARγ and PI3K/Akt signaling pathway related proteins in adipose tissue of the diabetic rats was analyzed by Western blotting. PTE treatment significantly reduced weight loss, FBG, insulin resistance, serum lipid levels and inflammatory factors. PTE treatment also inhibited oxidative stress by decreasing MDA expression and increasing SOD expression. Simultaneously, PTE treatment significantly ameliorated morphological impairment of the pancreas in diabetic rats. Furthermore, PTE treatment significantly increased the protein expression of PPARγ, PI3K, p-Akt, GLUT4 and IRS-1 in adipose tissues of diabetic rats. This study suggests that PTE can exert antidiabetic effects via the PI3K/Akt signaling pathway.
紫檀芪（PTE）是白藜芦醇的一种天然二甲化类似物，具有抗氧化、降血脂和降血糖作用；然而，其作用机制尚不清楚。在这项研究中，我们评估了 PTE 对糖尿病大鼠的作用，并阐明了其作用机制。糖尿病大鼠通过链脲佐菌素（STZ）和高糖高脂饮食诱导。然后，大鼠用 PTE（20、40 和 80mg/kg/d）治疗 8 周。在治疗结束时，通过口服葡萄糖耐量试验（OGTT）测量糖尿病大鼠的糖代谢。使用自动生化分析仪测定空腹血糖（FBG）、空腹胰岛素（FINS）和血脂谱，并通过酶联免疫吸附试验分析血清炎症因子。通过分光光度法分析血清超氧化物歧化酶（SOD）和丙二醛（MDA）也评估了抗氧化作用。通过 Western blot 分析糖尿病大鼠脂肪组织中过氧化物酶体增殖物激活受体γ（PPARγ）和 PI3K/Akt 信号通路相关蛋白的表达。PTE 治疗显著减轻了体重减轻、FBG、胰岛素抵抗、血清脂质水平和炎症因子。PTE 治疗还通过降低 MDA 表达和增加 SOD 表达抑制氧化应激。同时，PTE 治疗显著改善了糖尿病大鼠胰腺的形态损伤。此外，PTE 治疗显著增加了糖尿病大鼠脂肪组织中 PPARγ、PI3K、p-Akt、GLUT4 和 IRS-1 的蛋白表达。这项研究表明，PTE 可以通过 PI3K/Akt 信号通路发挥抗糖尿病作用。
