Molet Lucie, Girlich Delphine, Bonnin Rémy A, Proust Alexis, Bouligand Jérôme, Bachelerie Françoise, Hantz Sébastien, Deback Claire
Laboratoire de Virologie, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpitaux Universitaires Paris Sud, Hôpital Paul Brousse, Villejuif, France; INSERM UMR-996 « Inflammation, Chimiokines et Immunopathologies », Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculté de Médecine, Clamart, France.
EA7361 « Structure, Dynamics, Function and Expression of Broad-spectrum β-lactamases », Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculté de Médecine, Le Kremlin-Bicêtre, France.
Papillomavirus Res. 2019 Dec;8:100169. doi: 10.1016/j.pvr.2019.100169. Epub 2019 Jul 5.
The linear reverse blotting assays are valid methods for accurate human papillomavirus (HPV) typing required to manage women at risk of developing cervical cancer. However, some samples showed a positive signal in HPV lines but failed to display a positive signal in subsequent typing lines (designated as HPV-X), which indicate that certain types were not available on the respective typing blots. The aim of this study is to elucidate the types or variants of HPV through the high-throughput sequencing (HTS) of 54 ASCUS cervical samples in which the viruses remained untypeable with INNO LiPA HPV assays. Low-risk (LR)-HPV types (HPV6, 30, 42, 62, 67, 72, 74, 81, 83, 84, 87, 89, 90 and 114), high-risk (HR)-HPV35 and possibly (p)HR-HPV73 were detected among HPV-X. Individual multiple infections (two to seven types) were detected in 40.7% of samples. Twenty-two specimens contained variants characterised by 2-10 changes. HPV30 reached the maximal number of 17 variants with relative abundance inferior or equal to 2.7%. The presence of L1 quasispecies explains why linear reverse blotting assays fail when variants compete or do not match the specific probes. Further studies are needed to measure the LR-HPV quasispecies dynamics and its role during persistent infection.
线性反向印迹分析是对有患宫颈癌风险的女性进行准确的人乳头瘤病毒（HPV）分型所需的有效方法。然而，一些样本在HPV检测线上显示出阳性信号，但在随后的分型检测线上（标记为HPV-X）却未能显示出阳性信号，这表明相应的分型印迹上没有某些类型。本研究的目的是通过对54例非典型鳞状细胞（ASCUS）宫颈样本进行高通量测序（HTS）来阐明HPV的类型或变体，这些样本中的病毒用INNO LiPA HPV检测法无法分型。在HPV-X中检测到低风险（LR）-HPV类型（HPV6、30、42、62、67、72、74、81、83、84、87、89、90和114）、高风险（HR）-HPV35以及可能的（p）HR-HPV73。在40.7%的样本中检测到个体多重感染（两到七种类型）。22个样本含有以2至10个变化为特征的变体。HPV30的变体数量最多，达到17个，相对丰度低于或等于2.7%。L1准种的存在解释了为什么当变体竞争或与特异性探针不匹配时线性反向印迹分析会失败。需要进一步研究来测定LR-HPV准种动态及其在持续感染中的作用。
