Zemmyo Daiki, Miyata Shogo
Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Micromachines (Basel). 2019 Jul 6;10(7):455. doi: 10.3390/mi10070455.
The degeneration of adipocyte has been reported to cause obesity, metabolic syndrome, and other diseases. To treat these diseases, an effective evaluation and drug-screening system for adipocyte culture is required. The objective of this study is to establish an three-dimensional cell culture system to enable the monitoring of lipid accumulation by measuring electrical impedance, and to determine the relationship between the impedance and lipid accumulation of adipocytes cultured three dimensionally. Consequently, pre-adipocytes, 3T3-L1 cells, were cultured and differentiated to the adipocytes in our culture system, and the electrical impedance of the three-dimensional adipocyte culture at a high frequency was related to the lipid accumulation of the adipocytes. In conclusion, the lipid accumulation of adipocytes could be evaluated in real time by monitoring the electrical impedance during culture.
据报道，脂肪细胞的退化会导致肥胖、代谢综合征和其他疾病。为了治疗这些疾病，需要一种有效的脂肪细胞培养评估和药物筛选系统。本研究的目的是建立一种三维细胞培养系统，通过测量电阻抗来监测脂质积累，并确定三维培养的脂肪细胞的阻抗与脂质积累之间的关系。因此，在前脂肪细胞3T3-L1细胞在我们的培养系统中进行培养并分化为脂肪细胞，三维脂肪细胞培养物在高频下的电阻抗与脂肪细胞的脂质积累有关。总之，通过在培养过程中监测电阻抗，可以实时评估脂肪细胞的脂质积累。
