College of Pharmacy, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, 406-799 Incheon, Republic of Korea; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, 7-45, Songdo-dong, Yeonsu-gu, 406-840 Incheon, Republic of Korea.
Enzyme Microb Technol. 2013 Oct 10;53(5):302-6. doi: 10.1016/j.enzmictec.2013.07.001. Epub 2013 Jul 16.
Label-free and real-time monitoring of stem cells based on electrical impedance measurement is increasingly utilized for the quality control of the isolated stem cells to be used in stem cell-based tissue therapy or regenerative medicine. In spite of that the proliferative capacity and multipotency of stem cells are dependent on the type and age of the source tissue, however, the effect of the cell senescence on the impedance measurement of stem cells has not yet been studied. We investigated whether the senescence of adipose tissue-derived stem cells (ADSCs) can be detected by electrical impedance spectroscopy. For this, ADSCs at passage 9 and 31 were prepared and those genetic characteristics and growth kinetics were evaluated by quantitative polymerase chain reaction and cell counting. While the identified ADSCs were grown on the indium tin oxide electrodes, the impedance spectra were measured and interpreted by fitting analysis with an equivalent circuit model. ADSCs at passage 9 adhered on the electrode were small and spindle-shaped whereas the cells at passage 31 were flattened and larger than younger cells. At the beginning of culture time when the cell adhesion occurred, the resistance at 4.6kHz of passage 31 cells was higher than passage 9 due to the larger size of older cells. Afterwards, the value of passage 9 cells increased higher than passage 31, since younger cells proliferated more than old cells. Therefore, the impedance measurement could characterize the proliferative capacity of ADSCs during expanded culture.
基于电阻抗测量的无标记和实时监测正越来越多地用于隔离干细胞的质量控制，以用于基于干细胞的组织治疗或再生医学。尽管干细胞的增殖能力和多能性取决于来源组织的类型和年龄，但细胞衰老对干细胞阻抗测量的影响尚未得到研究。我们研究了脂肪组织来源的干细胞（ADSCs）的衰老是否可以通过电阻抗谱来检测。为此，制备了第 9 代和第 31 代 ADSCs，并通过定量聚合酶链反应和细胞计数评估这些遗传特征和生长动力学。当将鉴定的 ADSCs 种植在铟锡氧化物电极上时，通过使用等效电路模型进行拟合分析来测量和解释阻抗谱。第 9 代 ADSCs 在电极上黏附的细胞较小且呈纺锤形，而第 31 代的细胞则较平且比年轻细胞大。在细胞黏附发生的培养初期，由于较大的细胞尺寸，第 31 代细胞在 4.6kHz 处的电阻高于第 9 代细胞。随后，第 9 代细胞的值增加高于第 31 代细胞，因为年轻细胞比老细胞增殖更多。因此，阻抗测量可以表征 ADSCs 在扩展培养过程中的增殖能力。
