College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Food Res Int. 2019 Sep;123:355-363. doi: 10.1016/j.foodres.2019.05.003. Epub 2019 May 3.
The physicochemical properties, biological functions and microbial degradation of inulins differ according to their degree of polymerization. However, the relationship between inulin activities and its effect on gut microbiota remains unknown. In this study, high fat diet with inulin (1 or 5 g/kg·bw), either with short or long chains groups were administered to different groups of mice (n = 10) for 10 weeks in order to investigate the effect of inulin on the microbial diversity of the animals. Litchi pericarp procyanidins (LPPC) were used for comparison purposes. Furthermore, the lipid metabolism and key regulator genes in mice were determined. The results indicated that natural inulin (1 g/kg·bw) ingestion reduced the body weight of fat mice between week 6-9. Glutathione peroxidase (GSH-Px) activity in liver was remarkably higher after adding long chain inulin (5 g/kg·bw) compared to high-fat-diet mice. Moreover, high dose of natural inulin regulated malondialdehyde and advanced glycation end-products levels in mice liver. Likewise, the high dose of short-chain inulin increased sterol response element binding protein 1 (SREBP-1), β-Hydroxy β-methylglutaryl-CoA (HMG-CoA) and ATP-binding cassette transporter A1 (ABCA1) genetic expression. A significant change on the abundance of six genera in gut microbial profile suggested that inulin has the ability to modulate the lipid metabolism regardless of chain length, mainly due to its impact on colon microbiota variety.
菊粉的理化性质、生物学功能和微生物降解因聚合度而异。然而，菊粉的活性与其对肠道微生物群的影响之间的关系尚不清楚。在这项研究中，高脂肪饮食中添加了（1 或 5g/kg·bw）短链或长链菊粉，分别给不同组的小鼠（n=10）喂食 10 周，以研究菊粉对动物微生物多样性的影响。荔枝果皮原花青素（LPPC）被用作比较。此外，还测定了小鼠的脂质代谢和关键调节基因。结果表明，天然菊粉（1g/kg·bw）摄入可在第 6-9 周降低肥胖小鼠的体重。与高脂肪饮食组相比，添加长链菊粉（5g/kg·bw）后，肝脏中的谷胱甘肽过氧化物酶（GSH-Px）活性显著升高。此外，高剂量天然菊粉调节了小鼠肝脏中丙二醛和晚期糖基化终产物的水平。同样，短链菊粉的高剂量增加了固醇反应元件结合蛋白 1（SREBP-1）、β-羟-β-甲基戊二酰辅酶 A（HMG-CoA）和三磷酸腺苷结合盒转运蛋白 A1（ABCA1）的基因表达。肠道微生物群谱中六个属的丰度发生了显著变化，表明菊粉具有调节脂质代谢的能力，而与链长无关，主要是由于其对结肠微生物群多样性的影响。
