Velge-Roussel F, Verwaerde C, Grzych J M, Auriault A, Capron A
Centre d'immunologie et de biologie parasitaire, INSERM 167, Lille, France.
J Immunol. 1989 Apr 1;142(7):2527-32.
A rat IgE mAb specific for larval Ag (26 kDa, 56 kDa) has been shown to protect rats against Schistosoma mansoni infection. Immunizations of Lou/M rats performed with this IgE (Ab1) induced the production of antiidiotypic antibodies (Ab2). Moreover, after this Ab2 production, anti-antiidiotypic antibodies (Ab3) were revealed. The screening of Ab3 isotypes showed the presence of IgG Ab3 and more interestingly of IgE Ab3, i.e., the same isotype as Ab1. These IgE and IgG antibodies recognized predominantly the 26-kDa Ag and were cytotoxic for schistosomula in the presence of platelets for IgE Ab3 and eosinophils for IgG Ab3. Both IgE and IgG Ab3 conferred by passive transfer protective immunity to infected rats (up to 50%). Thus the immunization with an IgE mAb led in part to the production of Ab3 of the same isotype as Ab1. In conclusion, these results suggest that the isotype selection of the antibodies of the third generation (Ab3) might be influenced by the Ab1. The respective role of the idiotope and isotype of Ab1 in isotype regulation is discussed.
一种针对幼虫抗原（26 kDa、56 kDa）的大鼠IgE单克隆抗体已被证明能保护大鼠免受曼氏血吸虫感染。用这种IgE（Ab1）对Lou/M大鼠进行免疫可诱导抗独特型抗体（Ab2）的产生。此外，在产生这种Ab2后，还发现了抗抗独特型抗体（Ab3）。对Ab3同种型的筛选显示存在IgG Ab3，更有趣的是存在IgE Ab3，即与Ab1相同的同种型。这些IgE和IgG抗体主要识别26-kDa抗原，并且对于IgE Ab3在有血小板存在时以及对于IgG Ab3在有嗜酸性粒细胞存在时对血吸虫幼虫具有细胞毒性。通过被动转移，IgE和IgG Ab3都赋予感染大鼠保护性免疫（高达50%）。因此，用IgE单克隆抗体进行免疫部分导致产生与Ab1相同同种型的Ab3。总之，这些结果表明第三代抗体（Ab3）的同种型选择可能受Ab1影响。讨论了Ab1的独特位和同种型在同种型调节中的各自作用。
