Sun Pei-Yang, Cai Rong-Lin, Li Pei-Fang, Zhu Yan, Wang Tao, Wu Jie, Li Nan, Liu Hui, Chu Hao-Ran
Department of Encephalopathy, Second Affiliated Hospital of Anhui University of CM, Hefei 230061, China.
School of Acupuncture, Moxibustion and Tuina, Anhui University of CM.
Zhongguo Zhen Jiu. 2019 Jul 12;39(7):741-7. doi: 10.13703/j.0255-2930.2019.07.017.
To observe the effects on hippocampal neuronal structure and hippocampal monoamine neurotransmitters such as norepinephrine (NE), 5-hydroxytryptamine (5-HT) and dopamine (DA) with acupuncture for promoting the circulation of the governor vessel and regulating the mental state ( acupuncture) in rats with post-stroke depression and explore the underlying effect mechanism of acupuncture on post-stroke depression.
A total of 48 clean-grade SD rats were randomized into a sham-operation group, a model group, a Tongdu Tiaoshen acupuncture group and a non-meridian-non-acupoint group, 12 rats in each one. In the model group, the Tongdu Tiaoshen acupuncture group and the non-meridian-non-acupoint group, the compound method of cerebral medial arterial occlusion and the chronic unpredictable mild stress was adopted to prepare the model of post-stroke depression. In the sham-operation group, skin was opened and the fascia muscle was separated, after artery exposed, no ligation was operated and the incision was sutured directly. In the sham-operation group and the model group, no any intervention was performed. In the Tongdu Tiaoshen acupuncture group, acupuncture was applied to "Baihui" (GV 20), "Shuigou" (GV 26), "Shenting" (CV 24) and "Dazhui" (GV 14) for 40 min in each treatment and the needles were manipulated at the acupoints once in 20 min. The treatment was given once a day, 6 times a week for 4 weeks consecutively. In the non-meridian-non-acupoint group, the depression between the 3rd and 4th metatarsal bones on the dorsum of the forelimb was selected in intervention and the needling technique was same as the Tongdu Tiaoshen acupuncture group. The behavioral changes were observed after intervention in each group. Using transmission electron microscopy, the ultrastructure of hippocampal CA 1 neurons was observed in the rats of each group. Using high performance liquid chromatography, the contents of NE, 5-HT and DA were determined.
Compared with the sham-operation group, the Zea-longa neurobehavioral score was increased (<0.01), the sucrose water consumption was reduced (<0.01), the number of times of horizontal movement and vertical movement in open-field test were reduced (both <0.01), the damage of the ultrastructure of hippocampal CA 1 neurons was aggravated and the contents of NE, 5-HT and DA were reduced in the model group (<0.01). Compared with the model group, the Zea-Longa neurobehavioral score was reduced (<0.01), the sucrose water consumption was increased (<0.01), the number of times of horizontal movement and vertical movement in open-field test were increased (both <0.01), the damage of the ultrastructure of hippocampal CA 1 neurons was alleviated and the contents of NE, 5-HT and DA were increased in the Tongdu Tiaoshen acupuncture group (<0.01, <0.05). Compared with the non-meridian-non-acupoint group, the Zea-Longa neurobehavioral score was reduced (<0.01), the sucrose water consumption was increased (<0.01), the number of times of horizontal movement and vertical movement in open-field test were increased (both <0.01), the damage of the ultrastructure of hippocampal CA 1 neurons was alleviated and the contents of NE, 5-HT and DA were increased in the Tongdu Tiaoshen acupuncture group (<0.01, <0.05).
The acupuncture improves the behavior of the model rats with post-stroke depression, repairs hippocampal neuronal damage, which is probably related to the contents of hippocampal monoamine neurotransmitters (NE, 5-HT and DA).
观察针刺督脉通阳调神法对脑卒中后抑郁大鼠海马神经元结构及海马单胺类神经递质去甲肾上腺素（NE）、5-羟色胺（5-HT）和多巴胺（DA）的影响，探讨针刺治疗脑卒中后抑郁的潜在作用机制。
将48只清洁级SD大鼠随机分为假手术组、模型组、督脉通阳调神针刺组和非经非穴组，每组12只。模型组、督脉通阳调神针刺组和非经非穴组采用大脑中动脉闭塞复合慢性不可预测性温和应激法制备脑卒中后抑郁模型。假手术组切开皮肤，分离筋膜肌肉，暴露动脉后不进行结扎，直接缝合切口。假手术组和模型组不做任何干预。督脉通阳调神针刺组针刺“百会”（GV 20）、“水沟”（GV 26）、“神庭”（CV 24）和“大椎”（GV 14），每次留针40分钟，每20分钟行针1次。每天治疗1次，每周6次，连续治疗4周。非经非穴组选取前肢背侧第3、4跖骨间凹陷处进行干预，针刺手法同督脉通阳调神针刺组。观察各组干预后的行为学变化。采用透射电子显微镜观察各组大鼠海马CA1区神经元超微结构。采用高效液相色谱法测定NE、5-HT和DA含量。
与假手术组比较，模型组Zea-longa神经行为学评分升高（<0.01），糖水消耗减少（<0.01），旷场试验水平运动和垂直运动次数减少（均<0.01），海马CA1区神经元超微结构损伤加重，NE、5-HT和DA含量降低（<0.01）。与模型组比较，督脉通阳调神针刺组Zea-Longa神经行为学评分降低（<0.01），糖水消耗增加（<0.01），旷场试验水平运动和垂直运动次数增加（均<0.01），海马CA1区神经元超微结构损伤减轻，NE、5-HT和DA含量增加（<0.01，<0.05）。与非经非穴组比较，督脉通阳调神针刺组Zea-Longa神经行为学评分降低（<0.01），糖水消耗增加（<0.01），旷场试验水平运动和垂直运动次数增加（均<0.01），海马CA1区神经元超微结构损伤减轻，NE、5-HT和DA含量增加（<0.01，<0.05）。
针刺可改善脑卒中后抑郁模型大鼠的行为，修复海马神经元损伤，其机制可能与海马单胺类神经递质（NE、5-HT和DA）含量有关。
