Sun Pei-Yang, Li Pei-Fang, Wang Tao, Wu Jie, Li Nan, Liu Hui, Li A-Hui, Zhang Jing-Jing, Chu Hao-Ran
Department of Encephalopathy, Second Affiliated Hospital of Anhui University of CM, Hefei 230061, China.
Graduate School of Anhui University of CM.
Zhongguo Zhen Jiu. 2020 Nov 12;40(11):1205-10. doi: 10.13703/j.0255-2930.20200522-k0006.
To observe the improvement of acupuncture on depression-like behavior in rats with post-stroke depression (PSD), and to explore its possible mechanism.
A total of 48 SD rats were randomized into a sham-operation group, a model group, a Tongdu Tiaoshen group and a non-meridian-non-acupoint group, 12 rats in each group. In the the model group, the Tongdu Tiaoshen group and the non-meridian-non-acupoint group, PSD model was established by the compound method of cerebral medial arterial occlusion, chronic unpredictable mild stress and isolation. Acupuncture was applied at "Dazhui"(GV 14), "Shuigou"(GV 26), "Baihui"(GV 20) and "Shenting" (GV 24) in the Tongdu Tiaoshen group, and at the bilateral dorsum hollows between 3rd and 4th metatarsal bones of the forelegs in the non-meridian-non-acupoint group, needles were retained for 40 min in the both groups, once a day, 6 times a week for 4 weeks. Before model establishment and after intervention, the ethology indice of Zea Longa neurological behavioral score, open-field test and sucrose preference test were evaluated in the 4 groups. The levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), phosphorylated mammalian target of rapamycin (p-mTOR), Beclin1 and LC3B-Ⅱ/Ⅰin the CA1 region of hippocampus were detected by western blot. The expressions of Beclin1, LC3B-ⅡmRNA in the CA1 region of hippocampus were detected by real-time PCR.
After model established, compared with the sham-operation group, the Zea Longa neurological behavioral scores were increased (<0.05), the scores of horizontal and vertical motion of the open-field test and the consumption of sucrose solution were decreased in the model group, the Tongdu Tiaoshen group and the non-meridian-non-acupoint group (<0.05). After intervention, compared with the sham-operation group, the Zea Longa neurological behavioral score, the expression of Beclin1, LC3B-Ⅱ/Ⅰ and LC3B-Ⅱin the CA1 region of hippocampus were increased (<0.05), the scores of horizontal and vertical motion of the open-field test, the consumption of sucrose solution and the expression of PI3K, p-Akt and p-mTOR were decreased in the model group (<0.05). After intervention, compared with the model group and the non-meridian-non-acupoint group, the Zea Longa neurological behavioral score, the expression of Beclin1, LC3B-Ⅱ/Ⅰ and LC3B-Ⅱin the CA1 region of hippocampus were decreased (<0.05), the scores of horizontal and vertical motion of the open-field test, the consumption of sucrose solution and the expression of PI3K, p-Akt and p-mTOR were increased in the Tongdu Tiaoshen group (<0.05).
acupuncture can effectively improve the depression-like behavior in PSD rats, its mechanism may relate to the activation of PI3K/Akt/mTOR signaling pathway and the inhibition of hippocampal neuron autophagy.
观察针刺对脑卒中后抑郁（PSD）大鼠抑郁样行为的改善作用，并探讨其可能机制。
将48只SD大鼠随机分为假手术组、模型组、通督调神组和非经非穴组，每组12只。模型组、通督调神组和非经非穴组采用大脑中动脉闭塞、慢性不可预测性温和应激及隔离的复合方法建立PSD模型。通督调神组针刺“大椎”（GV 14）、“水沟”（GV 26）、“百会”（GV 20）和“神庭”（GV 24），非经非穴组针刺前肢第3、4跖骨间双侧背侧凹陷处，两组均留针40分钟，每日1次，每周6次，共4周。在造模前及干预后，对4组大鼠进行Zea Longa神经行为学评分、旷场试验和蔗糖偏好试验等行为学指标评估。采用蛋白质免疫印迹法检测海马CA1区磷脂酰肌醇3激酶（PI3K）、磷酸化蛋白激酶B（p-Akt）、磷酸化哺乳动物雷帕霉素靶蛋白（p-mTOR）、Beclin1和LC3B-Ⅱ/Ⅰ的水平。采用实时荧光定量PCR检测海马CA1区Beclin1、LC3B-ⅡmRNA的表达。
造模后，与假手术组比较，模型组、通督调神组和非经非穴组Zea Longa神经行为学评分升高（<0.05），旷场试验水平和垂直运动得分及蔗糖溶液消耗量降低（<0.05）。干预后，与假手术组比较，模型组Zea Longa神经行为学评分、海马CA1区Beclin1、LC3B-Ⅱ/Ⅰ和LC3B-Ⅱ表达升高（<0.05），旷场试验水平和垂直运动得分、蔗糖溶液消耗量及PI3K、p-Akt和p-mTOR表达降低（<0.05）。干预后，与模型组和非经非穴组比较，通督调神组Zea Longa神经行为学评分、海马CA1区Beclin1、LC3B-Ⅱ/Ⅰ和LC3B-Ⅱ表达降低（<0.05），旷场试验水平和垂直运动得分、蔗糖溶液消耗量及PI3K、p-Akt和p-mTOR表达升高（<0.05）。
针刺可有效改善PSD大鼠的抑郁样行为，其机制可能与激活PI3K/Akt/mTOR信号通路及抑制海马神经元自噬有关。
