Xiao Wei, Zhang Xianbao, Wang Zhen, Wang Yan, Guo Xiaoli, He Ling, Liang Fajun, Hu Huixing
The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.
Anhui University of CM.
Zhongguo Zhen Jiu. 2017 Jun 12;37(6):637-641. doi: 10.13703/j.0255-2930.2017.06.017.
To observe the effects of acupuncture on mRNA of the neurotransmitter serotonin transporter (5-HTT), 5-HTreceptor (5-HTR), norepinephrineα2 receptor (NEαR) in brain tissue in rats with post-stroke depression.
Forty male SD rats were randomly divided into a normal group, a model group, a medication group and an acupuncture group, 10 rats in each one. The model of post-stroke depression was established by occlusion of middle cerebral artery and chronic unpredictable mild stress method. After model was successfully established, the rats in the normal group and model group received no treatment; the rats in medication group were treated with intragastric administration of fluoxetine (2 mg/kg); the rats in acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Fengfu" (GV 16), "Shenmen" (HT 7) and "Taichong" (LR 3) for 20 min, during which manual stimulation was given once, once a day, 7 days as one course, and totally 3 courses were given with an interval of one day between courses. The changes of rat behavior and 5-HT, NE were observed after intervention; the RT-PCR method was applied to observe the mRNA of 5-HTT, 5-HTR, NEαR in hippocampus, raphe nucleus and locus coeruleus.
After treatment, compared with the normal group, the Zea Longa score in the model group was increased, while sugar water consumption, the number of horizontal and vertical movement of open-field test was reduced (all <0.01); compared with the model group, the Zea Longa score in the medication group and acupuncture group was reduced, while sugar water consumption, the number of horizontal and vertical movement of open-field test were increased (<0.01, <0.05); compared between the medication group and acupuncture group, the behavior changes were not significantly different (all >0.05). After treatment, compared with the normal group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HTR in hippocampus, raphe nucleus and locus coeruleus in the model group were reduced (all <0.01), but the mRNA expression of NEαR was increased (<0.01);compared with the model group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HTR in hippocampus, raphe nucleus and locus coeruleus in the medication group and acupuncture group were increased (<0.01, <0.05), while the mRNA expression of NEαR was reduced (all <0.01). The differences between medication group and acupuncture group were not significantly different (all >0.05).
Acupuncture could significantly improve behavioral change in rats with post-stroke depression, which may be related to the regulation of 5-HT, NE in cerebral cortex as well as mRNA expressions of 5-HTT, 5-HTR, NEαR in hippocampus, raphe nucleus and locus coeruleus.
观察针刺对脑卒中后抑郁大鼠脑组织中神经递质5-羟色胺转运体（5-HTT）、5-羟色胺受体（5-HTR）、去甲肾上腺素α2受体（NEαR）mRNA的影响。
将40只雄性SD大鼠随机分为正常组、模型组、药物组和针刺组，每组10只。采用大脑中动脉闭塞和慢性不可预测性轻度应激法建立脑卒中后抑郁模型。模型成功建立后，正常组和模型组大鼠不做处理；药物组大鼠灌胃给予氟西汀（2mg/kg）；针刺组大鼠针刺“百会”（GV 20）、“风府”（GV 16）、“神门”（HT 7）和“太冲”（LR 3）20分钟，期间手法捻转1次，每日1次，7天为1个疗程，共给予3个疗程，疗程间间隔1天。干预后观察大鼠行为及5-HT、NE的变化；采用RT-PCR法观察海马、中缝核和蓝斑中5-HTT、5-HTR、NEαR的mRNA。
治疗后，与正常组比较，模型组Zea Longa评分升高，糖水消耗量、旷场试验水平及垂直运动次数减少（均P<0.01）；与模型组比较，药物组和针刺组Zea Longa评分降低，糖水消耗量、旷场试验水平及垂直运动次数增加（P<0.01，P<0.05）；药物组与针刺组比较，行为学变化差异无统计学意义（均P>0.05）。治疗后，与正常组比较，模型组脑组织中5-HT、NE含量及海马、中缝核和蓝斑中5-HTT、5-HTR的mRNA表达降低（均P<0.01），但NEαR的mRNA表达升高（P<0.01）；与模型组比较，药物组和针刺组脑组织中5-HT、NE含量及海马、中缝核和蓝斑中5-HTT、5-HTR的mRNA表达升高（P<0.01，P<0.05），而NEαR的mRNA表达降低（均P<0.01）。药物组与针刺组比较，差异无统计学意义（均P>0.05）。
针刺可显著改善脑卒中后抑郁大鼠的行为变化，其机制可能与调节大脑皮质5-HT、NE以及海马、中缝核和蓝斑中5-HTT、5-HTR、NEαR的mRNA表达有关。
