YoungLai E V, Wilkinson M, Thompson N
Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ontario, Canada.
J Steroid Biochem. 1988 Mar;29(3):347-51. doi: 10.1016/0022-4731(88)90037-4.
The effects of an opioid antagonist, naloxone, on the secretion of gonadotrophins were investigated in the long term ovariectomized rabbit. In the intact and acutely ovariectomized rabbit (2 days p.o.) naloxone at 10 mg/kg induced an increase of 260-300% in LH secretion at 40 min post-injection. From days 33-66 post-surgery naloxone at 10 mg/kg caused significant elevations in LH release even when animals were treated with estradiol benzoate 24 h previously. By contrast, treatment with oestradiol benzoate 3 h before naloxone abolished the LH increase. An LH surge could be elicited in these rabbits with GnRH treatment. These studies indicated that long term ovariectomy in the female rabbit does not completely remove the opioid control of GnRH release and that the LH response to naloxone is influenced by circulating estradiol levels.
在长期卵巢切除的兔子中研究了阿片类拮抗剂纳洛酮对促性腺激素分泌的影响。在完整的和急性卵巢切除的兔子（术后2天）中，10mg/kg的纳洛酮在注射后40分钟可使促黄体生成素（LH）分泌增加260 - 300%。在术后33 - 66天，即使动物在24小时前用苯甲酸雌二醇治疗，10mg/kg的纳洛酮仍可导致LH释放显著升高。相比之下，在纳洛酮给药前3小时用苯甲酸雌二醇治疗可消除LH的增加。用促性腺激素释放激素（GnRH）治疗可在这些兔子中引发LH峰。这些研究表明，雌性兔子的长期卵巢切除并未完全消除阿片类物质对GnRH释放的控制，并且LH对纳洛酮的反应受循环雌二醇水平的影响。
