Younglai E V, Thompson N, Wilkinson M
Department of Obstetrics & Gynecology, McMaster University, Hamilton, Ontario, Canada.
Biol Reprod. 1988 Oct;39(3):630-7. doi: 10.1095/biolreprod39.3.630.
The influence of ovariectomy and steroid replacement on naloxone-induced changes in pulsatile secretion of luteinizing hormone (LH) in the female rabbit was examined. Blood samples were taken every 5 min through an indwelling catheter in the rabbit ear artery, and plasma was stored until assayed for LH by established radioimmunoassay procedures. In the intact animal, saline injection had no effect on LH secretion. Although naloxone (10 mg/kg) caused a 7-fold increase in mean LH pulse amplitude by 30 min after injection, this increase was not statistically significant because 5 of 11 animals did not respond. In animals ovariectomized 48 h previously, naloxone significantly increased LH concentration by 194% at 23 min after injection. When long-term ovariectomized rabbits were treated with estradiol benzoate and then were given naloxone, no significant increase in LH was observed, although many animals did respond. Treatment of long-term ovariectomized rabbits with 1 microgram estradiol benzoate and 100 micrograms progesterone or 1 mg testosterone propionate on Days 1 and 3 and naloxone on Day 4 resulted in a significant increase in LH 19-24 min later. Although there was an increase in pulse amplitude, no change was detected in pulse frequency after naloxone. These data suggest that the hypothesis of steroid-opioid coupling in the control of LH secretion is not applicable to the female rabbit.
研究了卵巢切除和类固醇替代对雌性兔中纳洛酮诱导的促黄体生成素（LH）脉冲分泌变化的影响。通过兔耳动脉中的留置导管每5分钟采集一次血样，血浆保存至通过既定的放射免疫分析程序检测LH。在完整动物中，注射生理盐水对LH分泌无影响。虽然纳洛酮（10mg/kg）在注射后30分钟使平均LH脉冲幅度增加了7倍，但由于11只动物中有5只无反应，这种增加在统计学上不显著。在48小时前进行卵巢切除的动物中，纳洛酮在注射后23分钟使LH浓度显著增加了194%。当长期卵巢切除的兔用苯甲酸雌二醇治疗后再给予纳洛酮时，虽然许多动物有反应，但未观察到LH有显著增加。在第1天和第3天用1微克苯甲酸雌二醇和100微克孕酮或1毫克丙酸睾酮治疗长期卵巢切除的兔，并在第4天给予纳洛酮，结果在19 - 24分钟后LH显著增加。虽然脉冲幅度有所增加，但纳洛酮后脉冲频率未检测到变化。这些数据表明，类固醇 - 阿片类物质偶联控制LH分泌的假说不适用于雌性兔。
