在巨大的单层囊泡内特异性 DNA 分子的等温扩增。
Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles.
机构信息
Department of Robotics, Tohoku University, Miyagi 980-8579, Japan.
出版信息
Chem Commun (Camb). 2019 Jul 30;55(62):9084-9087. doi: 10.1039/c9cc03277k.
PMID:31287464
Abstract
An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.
摘要
在巨大的单层囊泡中实现了特定 DNA 分子的等温扩增电路。利用该电路，实现了输出 DNA 的 5000 倍以上的扩增，并且扩增行为取决于细胞大小隔室中输入信号 DNA 的浓度。此外，还证明了通过光刺激引发扩增。
Zotero 插件