提议的 BoNT/A 和 /B 肽底物不能在 Endopep-MS 测定中检测多种亚型。
Proposed BoNT/A and /B Peptide Substrates Cannot Detect Multiple Subtypes in the Endopep-MS Assay.
机构信息
Division of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Buford Hwy, Northeast Atlanta, GA, USA.
Service de Pharmacologie et Immunoanalyse, Laboratoire d'Etude du Métabolisme des Médicaments, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut National de la Recherche Agronomique, Université Paris Saclay, Gif-sur-Yvette, France.
出版信息
J Anal Toxicol. 2020 Mar 7;44(2):173-179. doi: 10.1093/jat/bkz044.
Botulinum neurotoxins (BoNTs) are a family of protein toxins consisting of seven known serotypes (BoNT/A-BoNT/G) and multiple subtypes within the serotypes, and all of which cause the disease botulism-a disease of great public health concern. Accurate detection of BoNTs in human clinical samples is therefore an important public health goal. To achieve this goal, our laboratory developed a mass spectrometry-based assay detecting the presence of BoNT via its enzymatic activity on a peptide substrate. Recently, publications reported the use of new peptide substrates to detect BoNT/A and /B with improved results over other peptide substrates. However, the authors did not provide results of their peptide substrate on multiple subtypes of BoNT. In this work, we describe the results of testing the new substrates with multiple BoNT/A and /B subtypes and find that the substrates cannot detect many subtypes of BoNT/A and /B.
肉毒神经毒素（BoNTs）是由七种已知血清型（BoNT/A-BoNT/G）和血清型内的多种亚型组成的蛋白质毒素家族，所有这些都会导致疾病肉毒中毒——一种公众健康高度关注的疾病。因此，准确检测人类临床样本中的 BoNTs 是一个重要的公共卫生目标。为了实现这一目标，我们实验室开发了一种基于质谱的检测方法，通过其对肽底物的酶活性来检测 BoNT 的存在。最近，有出版物报道了使用新的肽底物来检测 BoNT/A 和 /B，其结果优于其他肽底物。然而，作者没有提供他们的肽底物在 BoNT 的多种亚型上的结果。在这项工作中，我们描述了用多种 BoNT/A 和 /B 亚型测试新底物的结果，发现这些底物不能检测许多亚型的 BoNT/A 和 /B。
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