Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77900 Olomouc, Czech Republic.
Molecules. 2019 Jul 8;24(13):2496. doi: 10.3390/molecules24132496.
In this study, we have compared four Ga-labeled peptides (three Arg-Gly-Asp (RGD) peptides and substance-P) with two F-tracers clinically approved for tumor imaging. We have studied in vitro and in vivo characteristics of selected radiolabeled tracers in a glioblastoma multiforme tumor model. The in vitro part of the study was mainly focused on the evaluation of radiotracers stability under various conditions. We have also determined in vivo stability of studied Ga-radiotracers by analysis of murine urine collected at various time points after injection. The in vivo behavior of tested Ga-peptides was evaluated through ex vivo biodistribution studies and PET/CT imaging. The obtained data were compared with clinically used F-tracers. Ga-RGD peptides showed better imaging properties compared to F-tracers, i.e., higher tumor/background ratios and no accumulation in non-target organs except for excretory organs.
在这项研究中，我们比较了四种 Ga 标记的肽（三种 Arg-Gly-Asp [RGD] 肽和 P 物质）与两种临床批准用于肿瘤成像的 F-示踪剂。我们在多形性胶质母细胞瘤肿瘤模型中研究了选定放射性示踪剂的体外和体内特性。研究的体外部分主要侧重于在各种条件下评估放射性示踪剂的稳定性。我们还通过分析注射后不同时间点收集的小鼠尿液来确定研究的 Ga 放射性示踪剂的体内稳定性。通过离体生物分布研究和 PET/CT 成像评估了测试的 Ga-肽的体内行为。获得的数据与临床使用的 F-示踪剂进行了比较。与 F-示踪剂相比，Ga-RGD 肽显示出更好的成像特性，即更高的肿瘤/背景比，并且除了排泄器官外，没有在非靶器官中积累。
