China Institute of Atomic Energy, P. O. Box 275 (78), Beijing 102413, China; Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China.
China Institute of Atomic Energy, P. O. Box 275 (78), Beijing 102413, China; HTA Co. LTD., Beijing 102413, China.
J Inorg Biochem. 2018 Sep;186:257-263. doi: 10.1016/j.jinorgbio.2018.06.004. Epub 2018 Jul 6.
With the advent of positron emission tomography/magnetic resonance imaging (PET/MRI) scanner, PET/MRI dual-modal imaging will play more and more important role in the diagnosis of cancers and other diseases. Until now, there is no an approved PET/MRI dual-modal imaging probe. The goal of this work is to design and synthesize potential PET/MRI dual-modal imaging probe based on superparamagnetic manganese ferrite nanoparticles. We have developed superparamagnetic nanoparticles that have uniform size with 5 nm and can be further functionalized through surface coating with dopamine and polyethylene glycol derivatives, which provide functional groups for conjugating tumor-targeting biomolecules and bifunctional chelators. The nanoparticles conjugated with integrin αβ over-expressed targeting cyclic arginine-glycine-aspartic acid (RGD)-peptide and labeled with positron radionuclide copper-64 were intravenously injected into glioblastoma xenograft nude mice. In vivo MRI and PET imaging of mice implied that the PET/MRI dual-modal imaging probe can precisely locate the tumor site with αβ over expression.
随着正电子发射断层扫描/磁共振成像（PET/MRI）扫描仪的出现，PET/MRI 双模成像将在癌症和其他疾病的诊断中发挥越来越重要的作用。到目前为止，还没有经过批准的 PET/MRI 双模成像探针。这项工作的目标是设计和合成基于超顺磁性锰铁氧体纳米粒子的潜在 PET/MRI 双模成像探针。我们已经开发出具有 5nm 均匀尺寸的超顺磁纳米粒子，并可以通过表面涂层进一步功能化，涂层材料是多巴胺和聚乙二醇衍生物，这些提供了用于连接肿瘤靶向生物分子和双功能螯合剂的功能基团。与整合素 αβ过表达靶向环精氨酸-甘氨酸-天冬氨酸（RGD）-肽连接并标记正电子放射性核素铜-64 的纳米粒子被静脉注射到胶质母细胞瘤异种移植裸鼠体内。对小鼠的体内 MRI 和 PET 成像表明，PET/MRI 双模成像探针可以精确定位 αβ过表达的肿瘤部位。
