Baba Y, Morita I, Susami T, Kuroda T, Murota S
Department of Orthodontics II, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Biochim Biophys Acta. 1988 May 2;960(1):67-72.
Myofibroblasts were cultured successfully from experimental wound tissue in rat palatal mucoperiosteum. Arachidonic acid metabolizing activity in cultured myofibroblasts was compared with that in fibroblasts cultured from normal mucoperiosteum. Prostaglandins biosynthesized from [14C]arachidonic acid in cell-free homogenates of both myofibroblasts and fibroblasts were prostaglandins D2, E2 and F2 alpha, and the activity producing each prostaglandin was not significantly different between the myofibroblasts and the fibroblasts, whereas smooth muscle cells, which are histologically similar to myofibroblasts, produced mainly 6-ketoprostaglandin F1 alpha, and relatively small amounts of prostaglandin E2. The release of arachidonic acid from cells prelabeled with [14C]arachidonic acid was compared among three types of cell. The calcium ionophore A23187 strongly enhanced arachidonic acid release in all three cell types. Bradykinin, 5-hydroxytryptamine and prostaglandin F2 alpha affected the stimulation of arachidonic acid release in the fibroblasts but were less or not effective in the myofibroblasts and smooth muscle cells. In addition, prostaglandin E2 biosynthesized in response to several stimuli was measured by radioimmunoassay. The content of prostaglandin E2 correlated closely with arachidonic acid release. In this study, we showed homogeneity between the myofibroblasts and fibroblasts in prostaglandin synthesizing activity and similarity in response to various stimuli between the myofibroblasts and smooth muscle cells, from the standpoint of arachidonic acid metabolism.
在大鼠腭部黏骨膜的实验性伤口组织中成功培养出了肌成纤维细胞。将培养的肌成纤维细胞中花生四烯酸的代谢活性与从正常黏骨膜培养的成纤维细胞中的代谢活性进行了比较。在肌成纤维细胞和成纤维细胞的无细胞匀浆中，由[14C]花生四烯酸生物合成的前列腺素为前列腺素D2、E2和F2α，并且肌成纤维细胞和成纤维细胞产生每种前列腺素的活性没有显著差异，而在组织学上与肌成纤维细胞相似的平滑肌细胞主要产生6-酮前列腺素F1α和相对少量的前列腺素E2。比较了三种细胞中预先用[14C]花生四烯酸标记的细胞释放花生四烯酸的情况。钙离子载体A23187在所有三种细胞类型中均强烈增强花生四烯酸的释放。缓激肽、5-羟色胺和前列腺素F2α影响成纤维细胞中花生四烯酸释放的刺激，但对肌成纤维细胞和平滑肌细胞的作用较小或无作用。此外，通过放射免疫测定法测量了对几种刺激作出反应而生物合成的前列腺素E2。前列腺素E2的含量与花生四烯酸的释放密切相关。在本研究中，从花生四烯酸代谢的角度来看，我们显示了肌成纤维细胞和成纤维细胞在前列腺素合成活性方面的同质性以及肌成纤维细胞和平滑肌细胞在对各种刺激的反应方面的相似性。
