Durant S, Duval D, Homo-Delarche F
Inserm U7, UA122, Hôpital Necker, Paris, France.
Prostaglandins Leukot Essent Fatty Acids. 1988 Jun;32(3):129-37.
In a model of mouse embryo fibroblasts in culture, which is characterized by a very rapid decrease in rate of cell proliferation during early passages, we determined the production of prostanoids either by following the transformation of the radioactive precursor [14C]-arachidonic acid or by radioimmunoassays. Our results demonstrate that mouse embryo fibroblasts in culture produce spontaneously substantial amounts of PGE2 and 6ketoPGF1 alpha as well as trace amounts of PGF2 alpha and of lipoxygenase derivatives (Hetes). We investigated the stability of the different types of prostaglandins produced. We showed that 6ketoPGF1 alpha and PGF2 alpha were stable in vitro, whereas PGE2 as a consequence of its solubilization in an aqueous medium was metabolized by 50% over a 24 h period, independently of the presence of the cell, thus leading to a constant underestimation of real PGE2 concentration. However, comparison of the patterns of prostaglandin production among subcultures of different orders was possible, and showed that the total amount of prostaglandin produced as well as the relative proportions are fairly identical during the first three passages, although the cell proliferation pattern rapidly decreases among the serial subcultures. These results suggest that prostaglandin production does not represent in our experimental model an autocrine means of regulating cell growth.
在一种体外培养的小鼠胚胎成纤维细胞模型中，其特点是在早期传代过程中细胞增殖速率迅速下降，我们通过追踪放射性前体[14C] - 花生四烯酸的转化或放射免疫测定来确定前列腺素的产生。我们的结果表明，体外培养的小鼠胚胎成纤维细胞自发产生大量的PGE2和6 - 酮 - PGF1α以及微量的PGF2α和脂氧合酶衍生物（Hetes）。我们研究了所产生的不同类型前列腺素的稳定性。我们发现6 - 酮 - PGF1α和PGF2α在体外是稳定的，而PGE2由于其在水性介质中的溶解，在24小时内有50%被代谢，与细胞的存在无关，从而导致对真实PGE2浓度的持续低估。然而，不同传代次数的亚培养物之间前列腺素产生模式的比较是可行的，并且表明在前三次传代期间，尽管在连续亚培养物中细胞增殖模式迅速下降，但所产生的前列腺素总量以及相对比例相当一致。这些结果表明，在我们的实验模型中，前列腺素的产生并不代表调节细胞生长的自分泌方式。
