Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Pharmacy Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Int J Radiat Biol. 2019 Nov;95(11):1462-1471. doi: 10.1080/09553002.2019.1642531. Epub 2019 Jul 23.
Protein kinase CK2 is a ubiquitously expressed kinase in eukaryotes, which is known to phosphorylate many protein substrates. Because CK2 is involved in the regulation of various signaling pathways, we wondered whether CK2 participated in the regulation of ionizing radiation (IR) induced biological process. In this study, we investigated the effect of IR on the subcellular localization and kinase activity in human non-small cell lung cancer (NSCLC) cells. Immunofluorescent results showed that CK2 subunits shuttle into the nucleus mostly beginning 1 h after IR and lasting more than 6 h. We also conducted in vitro kinase assay and observed an increase in CK2 kinase activity at 6 h after IR. Furthermore, an increase in S phase was observed at 6 h after IR. Colony formation assay results demonstrated that CK2 inhibitor CX-4945 significantly enhanced the effect of irradiation in NSCLC cells. These results indicated that CK2 may be implicated in the regulation of IR-induced biological process.
蛋白激酶 CK2 是真核生物中广泛表达的激酶，已知其可磷酸化许多蛋白质底物。由于 CK2 参与各种信号通路的调节，我们想知道 CK2 是否参与了电离辐射 (IR) 诱导的生物学过程的调节。在这项研究中，我们研究了 IR 对人非小细胞肺癌 (NSCLC) 细胞亚细胞定位和激酶活性的影响。免疫荧光结果表明，IR 后 1 小时内 CK2 亚基主要进入细胞核，并持续超过 6 小时。我们还进行了体外激酶测定，观察到 IR 后 6 小时 CK2 激酶活性增加。此外，IR 后 6 小时观察到 S 期增加。集落形成实验结果表明，CK2 抑制剂 CX-4945 显著增强了 NSCLC 细胞的辐射效应。这些结果表明 CK2 可能参与了 IR 诱导的生物学过程的调节。
