为什么对位氨基基团会使绿色荧光蛋白发色团失去荧光：相干的分子内电荷转移降低了 Z/E-光异构化势垒。
Why does a para-amino group make the green fluorescent protein chromophore non-fluorescent: coherent intramolecular charge transfer reduces the Z/E-photoisomerization barrier.
机构信息
Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
Faculty of Family Medicine, Northern Ontario School of Medicine, Ontario, Canada.
出版信息
Chem Commun (Camb). 2019 Aug 7;55(61):8991-8994. doi: 10.1039/c9cc04156g. Epub 2019 Jul 10.
A para-N-phenyl-amino group significantly increases the fluorescence quantum yield of N-phenyl-4-aminostilbene by the "amino conjugation effect", but, in contrast, a para-amino group in the para-amino analogue (p-ABDI) of the green fluorescent protein (GFP) chromophore makes p-ABDI non-fluorescent because the coherent photo-induced intramolecular charge transfer reduces the barrier of the Z/E-photoisomerization.
一个对- N - 苯氨基基团通过“氨基共轭效应”显著提高 N - 苯基-4-氨基苯乙烯的荧光量子产率，但相反，绿色荧光蛋白（GFP）发色团的对位氨基类似物（p-ABDI）中的对位氨基基团使 p-ABDI 无荧光，因为相干光诱导的分子内电荷转移降低了 Z / E-光异构化的势垒。
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