Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu 41944, South Korea.
Org Biomol Chem. 2019 Jul 24;17(29):7088-7094. doi: 10.1039/c9ob00948e.
Hydrogen sulfide (H2S) has been reported as a gaseous signaling molecule in cells. H2S modulation is dependent on the partial pressure of oxygen in cells, which means hypoxia can induce H2S production under various pathophysiological conditions. Hypoxia is a common condition in solid tumors and can lead to malignant tumors that may become aggressive and result in worse prognosis. We designed and synthesized probe Cu-CD for H2S detection under hypoxia conditions. It is selective and sensitive toward various biological thiols, reactive nitrogen species (RNS), and reactive oxygen species (ROS). The fluorescence intensity of Cu-CD in the cytoplasms of HeLa and EMT6 cells was enhanced in proportion to the concentration of exogenous/endogenous H2S. Moreover, Cu-CD can be able to detect endogenous H2S production accompanied by expression of HIF-1α. Therefore, Cu-CD can be a key tool to explore how H2S contributes to neovascularization and growth of solid tumor tissues in pathophysiological or hypoxic conditions.
硫化氢 (H2S) 已被报道为细胞中的气态信号分子。H2S 的调节依赖于细胞中的氧分压，这意味着在各种病理生理条件下缺氧可诱导 H2S 的产生。缺氧是实体瘤中的常见情况，可导致恶性肿瘤变得侵袭性更强，预后更差。我们设计并合成了探针 Cu-CD 用于检测缺氧条件下的 H2S。它对各种生物硫醇、活性氮物种 (RNS) 和活性氧物种 (ROS) 具有选择性和敏感性。HeLa 和 EMT6 细胞细胞质中 Cu-CD 的荧光强度与外源性/内源性 H2S 的浓度成正比增强。此外，Cu-CD 能够检测到伴随 HIF-1α 表达的内源性 H2S 产生。因此，Cu-CD 可以成为探索 H2S 如何在病理生理或缺氧条件下促进实体瘤组织新生血管形成和生长的关键工具。
