Department of Chemistry , National Institute of Technology (NIT) Kurukshetra , Kurukshetra - 136119 , Haryana , India.
Department of Science & Humanities , Karunya Institute of Technology & Sciences , Coimbatore 641114 , Tamil Nadu , India.
ACS Sens. 2018 Jun 22;3(6):1142-1148. doi: 10.1021/acssensors.8b00174. Epub 2018 Jun 13.
A new approach for the detection of hydrogen sulfide (HS) was constructed within vesicles comprising phospholipids and amphiphilic copper complex as receptor. 1,2-Distearoyl- sn-glycero-3-phosphocholine (DSPC) vesicles with embedded metal complex receptor (1.Cu) sites have been prepared. The vesicles selectively respond to HS in a buffered solution and show colorimetric as well as spectral transformation. Other analytes such as reactive sulfur species, reactive nitrogen species, biological phosphates, and other anions failed to induce changes. The HS detection is established through a metal indicator displacement (MIDA) process, where Eosin-Y (EY) was employed as an indicator. Fluorescence, UV-vis spectroscopy, and the naked eye as the signal readout studies confirm the high selectivity, sensitivity, and lower detection limit of the vesicular receptor. The application of vesicular receptors for real sample analysis was also confirmed by fluorescence live cell imaging.
一种新的用于检测硫化氢 (HS) 的方法是在包含磷脂和两亲性铜配合物作为受体的囊泡内构建的。已经制备了具有嵌入金属配合物受体 (1.Cu) 位点的 1,2-二硬脂酰基-sn-甘油-3-磷酸胆碱 (DSPC) 囊泡。在缓冲溶液中，囊泡选择性地响应 HS，并且显示出比色和光谱转变。其他分析物，如活性硫物种、活性氮物种、生物磷酸盐和其他阴离子，都不能引起变化。HS 的检测是通过金属指示剂置换 (MIDA) 过程建立的，其中曙红 Y (EY) 被用作指示剂。荧光、紫外-可见光谱和肉眼作为信号读出研究证实了囊泡受体的高选择性、灵敏度和更低的检测限。通过荧光活细胞成像也证实了囊泡受体在实际样品分析中的应用。
