Sarban Sezgin, Tabur Hasan, Baba Z Füsun, Işıkan U Erdem
Department of Orthopedics and Traumatology, Acıbadem Mehmet Ali Aydınlar University, 34752 Ataşehir, İstanbul, Turkey.
Eklem Hastalik Cerrahisi. 2019 Aug;30(2):91-6. doi: 10.5606/ehc.2019.64018.
This study aims to investigate the potential use and histological effects of the local administration of platelet-derived growth factor (PDGF) in the repair of full-thickness osteochondral defects in articular cartilage in an animal model.
Twenty-four adolescent 18-week-old New Zealand White rabbits with an average weight of 2500 g (range, 1600 g to 3200 g) were used in the study. The rabbits were randomly divided into three groups (n=8) as the control group (group A) and two experimental groups (groups B and C). Defects of cylindrical full-thickness (3.5 mm wide, 4 mm deep) were created in the weight-bearing area of the right knee medial femoral condyles. In group A, the defect was left empty. In group B, the defect was filled with a collagen sponge. In group C, the defect was filled with a collagen sponge impregnated with PDGF. All rabbits were followed-up for 12 weeks. Right knee medial femoral condyles were used for macroscopic and histological analyses.
At macroscopic level, the repair tissue was similar to normal adjacent cartilage at 12 weeks in group C. The surface of the repair tissue in group C was smoother and more regular compared to groups A and B. The total histological score of defects in group C was statistically significantly superior compared to groups A and B (p<0.05). Matrix staining and immunostaining of collagen type 2 were stronger in group C compared to the other groups, indicating the presence of a tissue similar to a normal cartilage.
Platelet-derived growth factor can induce repair in full-thickness defects of articular cartilage in an animal model. Thus, this study demonstrates the potential use of PDGF for full-thickness osteochondral defects.
本研究旨在探讨在动物模型中局部应用血小板衍生生长因子（PDGF）修复关节软骨全层骨软骨缺损的潜在用途及组织学效果。
本研究使用了24只18周龄的青春期新西兰白兔，平均体重2500克（范围为1600克至3200克）。将兔子随机分为三组（n = 8），即对照组（A组）和两个实验组（B组和C组）。在右膝内侧股骨髁的负重区域制造圆柱形全层缺损（宽3.5毫米，深4毫米）。A组缺损处不做处理；B组缺损处填充胶原海绵；C组缺损处填充含PDGF的胶原海绵。所有兔子随访12周。取右膝内侧股骨髁进行宏观和组织学分析。
在宏观水平上，C组在12周时修复组织与相邻正常软骨相似。与A组和B组相比，C组修复组织表面更光滑、更规则。C组缺损的组织学总评分在统计学上显著优于A组和B组（p < 0.05）。与其他组相比，C组的基质染色和Ⅱ型胶原免疫染色更强，表明存在类似于正常软骨的组织。
血小板衍生生长因子可在动物模型中诱导关节软骨全层缺损的修复。因此，本研究证明了PDGF在全层骨软骨缺损中的潜在用途。
