Associate Professor, Department of Oncology, Radiotherapy and Plastic Surgery; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8/2 Trubetskaya St., Moscow, 119991, Russia.
Junior Researcher, Department of Oncology, Radiotherapy and Plastic Surgery; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8/2 Trubetskaya St., Moscow, 119991, Russia.
Sovrem Tekhnologii Med. 2021;12(6):54-60. doi: 10.17691/stm2020.12.6.07. Epub 2020 Dec 28.
Platelet-derived growth factor (PDGF) plays an important role in angiogenesis, affects activation of migration and proliferation of mesenchymal stem cells, fibroblasts, smooth muscle cells, osteoblasts; activation of migration of monocytes, macrophages, and neutrophils. was to study the effect of cryo-processing on the qualitative properties of platelet-rich autoplasma (PRP) at different time intervals.
Autologous plasma preparations were obtained from the blood of 31 donors. The biological material was prepared by double centrifugation according to the protocol for obtaining P-PRP and L-PRP. Platelet count and the concentration of growth factors (PDGF-AA and PDGF-BB) were studied in fresh PRP preparations. In frozen PRP samples, the concentration of PDGF-AA and PDGF-BB was determined 2 weeks after cryo-processing and 2 months after cryo-processing at -35 °С. P-PRP and L-PRP samples activated with 10% CaCl solution and those non-activated were studied.
L-PRP preparations are significantly superior to P-PRP preparations: the concentration of platelets is 1.7 times higher in them. The level of PDGF-AA in non-activated L-PRP is 1.8 times higher than in non-activated P-PRP (p<0.05). The level of PDGF-AA is 1.5 times higher in activated L-PRP than in activated P-PRP (p<0.05). The level of PDGF-BB is 2.9 times higher in non-activated L-PRP than in non-activated P-PRP and 1.8 times higher in activated L-PRP than in activated P-PRP (p<0.05). The concentration of PDGF-BB in non-activated P-PRP sharply increases in the 2 week after freezing and remains at the same level after 2 months (p<0.05). The concentration of PDGF-BB in activated plasma does not change (p>0.05).
Cryo-processing of non-activated autologous L-PRP allows preserving and subsequently enhancing the properties of plasma concentrate, which makes it possible to apply it in clinical practice.
血小板衍生生长因子 (PDGF) 在血管生成中起重要作用，影响间充质干细胞、成纤维细胞、平滑肌细胞、成骨细胞的迁移和增殖的激活；单核细胞、巨噬细胞和嗜中性粒细胞的迁移激活。目的是研究不同时间间隔下冷冻处理对富含血小板自血浆 (PRP) 定性特性的影响。
从 31 名供体的血液中获得自体血浆制剂。生物材料按获得 P-PRP 和 L-PRP 的方案通过双离心法制备。研究新鲜 PRP 制剂中血小板计数和生长因子（PDGF-AA 和 PDGF-BB）的浓度。在冷冻 PRP 样品中，在 -35°C 下冷冻处理 2 周和 2 个月后测定 PDGF-AA 和 PDGF-BB 的浓度。研究了用 10%CaCl 溶液激活的 P-PRP 和 L-PRP 样本以及未激活的样本。
L-PRP 制剂明显优于 P-PRP 制剂：其中血小板浓度高 1.7 倍。非激活的 L-PRP 中的 PDGF-AA 水平比非激活的 P-PRP 高 1.8 倍（p<0.05）。激活的 L-PRP 中的 PDGF-AA 水平比激活的 P-PRP 高 1.5 倍（p<0.05）。非激活的 L-PRP 中的 PDGF-BB 水平比非激活的 P-PRP 高 2.9 倍，激活的 L-PRP 中的 PDGF-BB 水平比激活的 P-PRP 高 1.8 倍（p<0.05）。非激活的 P-PRP 中的 PDGF-BB 浓度在冷冻后 2 周内急剧增加，并在 2 个月后保持相同水平（p<0.05）。激活后的血浆中 PDGF-BB 的浓度没有变化（p>0.05）。
非激活自体 L-PRP 的冷冻处理可以保存和随后增强血浆浓缩物的特性，使其有可能在临床实践中应用。
