Nath Mousumi, Chowdhury Farhana Tasnim, Ahmed Shabbir, Das Avizit, Islam Mohammad Riazul, Khan Haseena
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
University of Science and Technology Chittagong, Chattogram, 4202, Bangladesh.
Heliyon. 2021 Mar 8;7(3):e06353. doi: 10.1016/j.heliyon.2021.e06353. eCollection 2021 Mar.
In the backdrop of an abundance of lignin in jute, the main focus of the present study was to conduct a quality assessment of four delignified jute lines (in which four lignin biosynthetic genes were individually downregulated) across advanced generations for industrial applications. To this end, the transgenic lines were advanced to 7 (COMT and C4H lines) and 5 (C3H and F5H lines) transformed generations. The results exhibit approximately 16-25% reduction in acid-insoluble lignin for the whole stem and 13-14% reduction in fiber lignin content for all four transgenic lines compared to the control. The altered lignin composition led to a 3-6% increase in the cellulose content and a small improvement in the enzymatic release of glucose. Lignin reduction led to an exposure of the underlying fibrils in transgenic lines as observed through a scanning electron microscope whereas, it was undiscernible in the control fiber. Furthermore, an analysis of the mechanical properties appears almost similar to that of the control with no morphological deformities. Jute fibers from the transgenic lines offer tremendous cost-effective implications from an economic perspective.
在黄麻中木质素含量丰富的背景下，本研究的主要重点是对四个脱木质素黄麻品系（其中四个木质素生物合成基因分别被下调）在多个世代进行质量评估，以用于工业应用。为此，转基因品系分别推进到了转化后的第7代（COMT和C4H品系）和第5代（C3H和F5H品系）。结果显示，与对照相比，所有四个转基因品系的全茎酸不溶性木质素含量降低了约16 - 25%，纤维木质素含量降低了13 - 14%。木质素组成的改变导致纤维素含量增加了3 - 6%，葡萄糖的酶促释放也有小幅改善。通过扫描电子显微镜观察发现，木质素减少导致转基因品系中潜在的原纤维暴露，而在对照纤维中则无法辨别。此外，对机械性能的分析显示与对照几乎相似，没有形态畸形。从经济角度来看，转基因品系的黄麻纤维具有巨大的成本效益优势。
