CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience , National Center for Nanoscience and Technology of China , Beijing 100190 , China.
School of Nanoscience and Technology, Sino-Danish College , University of Chinese Academy of Sciences , Beijing 100049 , China.
Anal Chem. 2019 Aug 6;91(15):9640-9647. doi: 10.1021/acs.analchem.9b01061. Epub 2019 Jul 24.
The T-plastin (PLS3) has a significant implication in epithelial-mesenchymal transition (EMT) and breast cancer prognosis. Using one-bead-one-compound library strategy, a novel peptide TP1 (KVKSDRVC) toward PLS3 was screened and exhibited the specificity for identifying PLS3-expressed cancer cells. Moreover, we found Fluorescein isothiocyanate-labeled TP1 (FITC-TP1) could act as a novel probe for EMT-induced cancer cells, preferentially in the leading edge. It also has satisfactory specificity for PLS3-expressed cancer cells spiked in the blood. FITC-TP1 was expected to become a diagnostic tool to identify PLS3-expressed circulating tumor cells and predict prognosis for patients with breast cancer in the future.
T 型丝切蛋白（PLS3）在上皮-间质转化（EMT）和乳腺癌预后中有重要意义。本研究采用单珠单化合物文库策略，筛选出一种针对 PLS3 的新型肽 TP1（KVKSDRVC），并表现出识别 PLS3 表达癌细胞的特异性。此外，我们发现异硫氰酸荧光素标记的 TP1（FITC-TP1）可作为 EMT 诱导的癌细胞的新型探针，优先在前沿部位发挥作用。它对血液中掺入的 PLS3 表达癌细胞也具有令人满意的特异性。FITC-TP1 有望成为一种诊断工具，用于识别 PLS3 表达的循环肿瘤细胞，并预测乳腺癌患者的预后。
