Genetics of Female Infertility: Molecular Study of Newborn Ovary Homeobox Gene in Poor Ovarian Responders.

BACKGROUND

Newborn ovary homeobox () gene plays a critical role in the transcriptional regulation of oocyte-specific genes. Previous studies have demonstrated a pathogenic effect of variants on premature ovarian insufficiency (POI) patients. Poor ovarian response (POR) is a risk factor for POI. Therefore, genetic variants in the gene may also be studied as risk factors for POR development.

AIMS

The aim of the study is to investigate the association between seven known single-nucleotide polymorphisms (SNPs) and POR in Jordanian females.

SETTINGS AND DESIGN

This was a case-control study of 60 females with POR for controlled ovarian hyperstimulation and 59 healthy females with no history of reproductive problems. Blood samples were collected from the participants and seven SNPs of gene were screened.

SUBJECTS AND METHODS

DNA was extracted from blood samples. Polymerase chain reaction with primers specific for seven known SNPs in gene was used to amplify the specified region within the gene followed by Sanger sequencing.

RESULTS

The seven SNPs investigated in this study, namely, rs77587352 (c.271G>T, p. Gly91Trp), rs7800847 (c.349C>T, p. Arg117Trp), rs193303102 (c.907C>T, p. Arg303X), rs193303103 (c.1025G>C, p. Ser342Thr), rs193303104 (c.1048G>T, p. Val350Leu), rs201947677 (c.1064G>A, p. Arg355His), and rs146227301 (c.1856C>T, p. Pro619Leu), only represent the wild-type allele in both females with POR and healthy participants.

CONCLUSIONS

The results show that only monomorphic genotype of the variants was found in Jordanian females studied.