Huang Jiajin, Ruan Yong, Xiao Meimei, Dai Lingang, Jiang Chuanmei, Li Jifeng, Xu Jiali, Chen Xiang, Xu Houqiang
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China.
Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China.
Front Vet Sci. 2024 Mar 8;11:1359312. doi: 10.3389/fvets.2024.1359312. eCollection 2024.
The newborn ovary homeobox gene () regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that is preferentially expressed in the ovary. Six mutations were detected in the sequence, including g.1624 T>C, g.1858 G>A, g.2770 G>A, g.2821 A>G, g.5659 A>G, and g.6025 T>A, of which g.1858 G>A was a missense mutation. However, only g.1858 G>A, g.5659 A>G, and g.6025 T>A were significantly associated with litter size traits ( < 0.05). Further prediction of the effect of the missense mutation g.1858 G>A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding.
新生卵巢同源盒基因（）调控卵巢和早期卵母细胞发育，因此在生殖过程中发挥着至关重要的作用。本研究对香猪各组织中的mRNA表达水平和单核苷酸多态性（SNP）进行了研究，以探讨其多态性与产仔数性状之间的关系。此外，利用生物信息学评估错义替换对蛋白质结构和功能的影响。结果显示，在卵巢中优先表达。在序列中检测到6个突变，包括g.1624 T>C、g.1858 G>A、g.2770 G>A、g.2821 A>G、g.5659 A>G和g.6025 T>A，其中g.1858 G>A为错义突变。然而，只有g.1858 G>A、g.5659 A>G和g.6025 T>A与产仔数性状显著相关（<0.05）。对错义突变g.1858 G>A对蛋白质功能影响的进一步预测表明，p.V82M是一个非保守突变，显著降低了蛋白质稳定性，从而改变了蛋白质功能。总体而言，这些发现表明多态性与香猪的产仔数密切相关，这可能为猪的育种提供新的见解。
