Hachemi Maroua, Bensaada Mustapha, Rouabah Abdelkader, Zoghmar Abdelali, Benbouhedja Sebti, Rouabah Leila, Benchaib Mehdi
Faculty of Nature and Life Sciences, Laboratory of Molecular and Cellular Biology, Frères Mentouri University Constantine I, Constantine, Algeria.
Reproduction Sciences and Surgery Clinique, Ibn Rochd, Constantine, Algeria.
J Hum Reprod Sci. 2019 Apr-Jun;12(2):122-129. doi: 10.4103/jhrs.JHRS_81_18.
Our study defines the clinical role of sperm DNA damage in the assisted reproductive technology procedure.
To investigate if the compaction of chromatin explored added to the analysis of the sperm DNA fragmentation allows obtaining a new indicator for sperm genome quality linked to live birth rate (LBR).
This was a prospective study, undergoing 101 cycles in the intracytoplasmic sperm injection (ICSI) program.
The sperm DNA fragmentation index (DFI) has been measured with sperm chromatin dispersion examination. The sperm decondensation index (SDI) of chromatin has been measured with aniline blue procedure; with these indexes, a new parameter has been created: DFI × SDI.
Pearson's correlation coefficient, Student's -test, and Chi-square test were used. The quantitative variables were described as mean ± standard deviation. Multivariate logistic regressions were performed with live birth as outcome.
The sperm concentration, motility, and normal morphology were lower when the DFI was high ( = 0.001). The fertilization rates and the number of obtained embryos were not statistically significant different according to the DFI groups. The SDI does not appear to be linked either with the spermatic parameters or with the ICSI parameters. A low DFI seems to be a beneficial factor to obtain a live birth in ICSI procedure ( = 0.064). In case of high DFI, a high SDI allows to obtain a higher LBR than a low SDI.
The DFI is a good prognostic for a delivery rate in ICSI procedure, and the SDI could be added to DFI to create a new parameter of sperm nuclear quality. This new parameter seems to be linked to LBR.
我们的研究确定了精子DNA损伤在辅助生殖技术过程中的临床作用。
探讨在精子DNA片段化分析中加入染色质凝聚分析是否能获得与活产率（LBR）相关的精子基因组质量新指标。
这是一项前瞻性研究，在卵胞浆内单精子注射（ICSI）项目中进行了101个周期。
采用精子染色质扩散试验检测精子DNA碎片指数（DFI）。采用苯胺蓝法检测染色质的精子解聚指数（SDI）；利用这些指标创建了一个新参数：DFI×SDI。
采用Pearson相关系数、Student's -检验和卡方检验。定量变量以均值±标准差描述。以活产为结局进行多因素逻辑回归分析。
DFI高时，精子浓度、活力和正常形态较低（P = 0.001）。根据DFI分组，受精率和获得的胚胎数量在统计学上无显著差异。SDI似乎与精子参数或ICSI参数均无关联。低DFI似乎是ICSI过程中获得活产的有利因素（P = 0.064）。在DFI高的情况下，高SDI比低SDI能获得更高的LBR。
DFI对ICSI过程中的分娩率是一个良好的预后指标，SDI可与DFI相加创建一个精子核质量新参数。这个新参数似乎与LBR相关。
