Sadeghi Mohamad Reza, Hodjat Mahshid, Lakpour Niknam, Arefi Soheila, Amirjannati Naser, Modarresi Tahereh, Jadda Hossain Hossaini, Akhondi Mohammad Mehdi
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Avicenna J Med Biotechnol. 2009 Oct;1(3):173-80.
Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opportunity to fertilize the egg which may lead to decreased embryo quality and implantation rate. The aim of this study was to evaluate the effects of sperm chromatin integrity on ICSI outcomes. A total of 200 semen samples were collected from couples undergoing ICSI and were analyzed according to WHO criteria. Each sample was evaluated for sperm chromatin integrity using four cytochemical assays and semen processing by swim up method. The ICSI was carried out according to a long-term pituitary down-regulation protocol. The correlation between sperm parameters, sperm chromatin integrity and ICSI outcomes (fertilization rate and embryo quality) was examined. The mean number of oocyte, fertilization rate and cleavage embryos per cycles was 7.5±5.0, 74.06%±25 and 5.4±3.6, respectively. There was not significant correlation between the results of chromatin assays (AO, AB, TB, and CMA3) and fertilization outcomes following ICSI. The fertilization rate was significantly higher for a group with less than 10% chromatin abnormality (p<0.05). Sperm chromatin integrity is essential for successful fertilization, embryo development and normal pregnancy. A protamine deficiency appeared to affect fertilization rate and embryo quality. However, the presence of confounding factors such as selection of spermatozoa according to normal morphology may influence the effect of sperm chromatin status on ICSI outcomes.
精子染色质完整性一直被视为男性生育能力的一个重要因素。在正常受精过程中，染色质完整的高质量精子在从阴道到输卵管的行程中通过自然选择被筛选出来。然而，使用辅助生殖技术，尤其是卵胞浆内单精子注射（ICSI）时，自然选择过程被绕过。因此，存在DNA断裂的精子有机会使卵子受精，这可能导致胚胎质量下降和着床率降低。本研究的目的是评估精子染色质完整性对ICSI结局的影响。从接受ICSI的夫妇中总共收集了200份精液样本，并根据世界卫生组织标准进行分析。使用四种细胞化学检测方法对每个样本的精子染色质完整性进行评估，并采用上游法进行精液处理。ICSI按照长期垂体降调节方案进行。检测精子参数、精子染色质完整性与ICSI结局（受精率和胚胎质量）之间的相关性。每个周期的平均卵母细胞数量、受精率和卵裂胚胎数分别为7.5±5.0、74.06%±25和5.4±3.6。染色质检测结果（吖啶橙、苯胺蓝、硫堇和CMA3）与ICSI后的受精结局之间没有显著相关性。染色质异常低于10%的组受精率显著更高（p<0.05）。精子染色质完整性对于成功受精、胚胎发育和正常妊娠至关重要。鱼精蛋白缺乏似乎会影响受精率和胚胎质量。然而，诸如根据正常形态选择精子等混杂因素的存在可能会影响精子染色质状态对ICSI结局的影响。
