Takeuchi M, Kakiuchi T, Taira S, Nariuchi H
Department of Allergology, University of Tokyo, Japan.
Immunobiology. 1987 Dec;176(1-2):1-13. doi: 10.1016/S0171-2985(87)80095-5.
Accessory cell (AC) function of B cells was examined in Con A response of a cloned T cell line, 22-9D, which is Thy 1+,L3T4+,Lyt2-,H-2KbDb+ and I-Ab-.22-9D cells produced IL 2 in the presence of Con A without participation of AC. For the initiation of a proliferative response to Con A, the addition of spleen cells or spleen adherent cells was required. B cells as AC were unable to induce the proliferative response. In the presence of culture supernatant of spleen cells stimulated with Con A (CAS), 22-9D cells showed proliferative response to Con A with B cell AC. The response was inhibited by a relevant monoclonal anti-I-A antibody. Although irradiated spleen cells as AC induced IL 2 receptor expression of 22-9D cells in the presence of Con A, B cells were shown to require the addition of unknown factor(s) in CAS, which was suggested to be different from IL 1, IL 2, IL 3, or IFN-gamma, for the induction of the receptor expression on 22-9D cells.
在一个克隆的T细胞系22 - 9D的刀豆蛋白A（Con A）反应中检测了B细胞的辅助细胞（AC）功能。该T细胞系具有以下特征：Thy 1阳性、L3T4阳性、Lyt2阴性、H - 2KbDb阳性且I - Ab阴性。22 - 9D细胞在有Con A存在时能产生白细胞介素2（IL 2），且无需辅助细胞参与。对于启动对Con A的增殖反应，需要添加脾细胞或脾黏附细胞。作为辅助细胞的B细胞无法诱导增殖反应。在经Con A刺激的脾细胞培养上清液（CAS）存在的情况下，22 - 9D细胞与B细胞辅助细胞一起对Con A表现出增殖反应。该反应被相关的抗I - A单克隆抗体抑制。尽管经辐照的脾细胞作为辅助细胞在有Con A存在时能诱导22 - 9D细胞表达IL 2受体，但已表明B细胞需要在CAS中添加未知因子才能诱导22 - 9D细胞表达该受体，推测该未知因子不同于IL 1、IL 2、IL 3或干扰素 - γ。
