Yamada A, Himeno K, Nakamura S, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunobiology. 1987 Dec;176(1-2):24-34. doi: 10.1016/S0171-2985(87)80097-9.
We found an antigen-specific factor capable of augmenting delayed-type hypersensitivity (DTH) in the culture supernatant of the mixture of immune T cells and specific antigen, or in the serum of mice immunized with xenogeneic erythrocytes and elicited for DTH footpad reaction. Previous experiments on the genetic restriction of this factor (DTH-augmentation factor; DAF) indicated that DAF activity was effective across the MHC-barrier in C3H/He (H-2k)--BALB/c (H-2d) system. The genetic restriction between DAF and its acceptor cells was then investigated precisely using Igh (immunoglobulin heavy chain locus)-congeneic mice: 1) Expression of DAF activity was MHC-nonrestricted, 2) but was restricted by the Igh-linked gene on the 12th chromosome, 3) such Igh-linked gene restriction was also demonstrated by an absorption test with normal spleen cells. The acceptor cells for DAF were Thy-1+,L3T4+,Lyt-2- T cells.
我们在免疫T细胞与特异性抗原混合物的培养上清液中，或在用异种红细胞免疫并引发迟发型超敏反应足垫反应的小鼠血清中，发现了一种能够增强迟发型超敏反应（DTH）的抗原特异性因子。此前关于该因子（DTH增强因子；DAF）遗传限制的实验表明，在C3H/He（H-2k）-BALB/c（H-2d）系统中，DAF活性在主要组织相容性复合体（MHC）屏障间有效。随后，使用免疫球蛋白重链基因座（Igh）同源小鼠精确研究了DAF与其受体细胞之间的遗传限制：1）DAF活性的表达不受MHC限制；2）但受第12号染色体上与Igh连锁的基因限制；3）通过用正常脾细胞进行的吸收试验也证实了这种与Igh连锁的基因限制。DAF的受体细胞是Thy-1+、L3T4+、Lyt-2-T细胞。
