Nakamura S, Himeno K, Yamada A, Kawamura I, Nomoto K
Cell Immunol. 1987 Mar;105(1):54-64. doi: 10.1016/0008-8749(87)90055-4.
We found an antigen-specific factor capable of augmenting delayed-type hypersensitivity (DTH) in the serum of mice sensitized with heterologous erythrocytes to induce a delayed footpad reaction (DFR), or in the culture supernatant of the mixture of sensitized T cells and specific antigens. This factor (DTH augmentation factor; DAF) was confirmed to augment DTH in transferred recipients. In this paper, such an activity of DAF was further investigated using the system with in vitro induction and local transfer of DTH. DAF also augmented the primary in vitro induction of DTH, when spleen cells from mice transferred with the DAF-containing serum 12 hr previously or spleen cells incubated with the DAF-containing serum on ice for 2 hr were cultured with heterologous erythrocytes. DAF acted on the induction phase of DTH and augmented a typical DTH which was dependent on Thy-1-positive T cells. DAF showed antigen specificity, but was not assigned to conventional immunoglobulin. The activity of DAF was detected when nylon-wool nonadherent cells were incubated with DAF prior to the culture of those cells and antigens, but not detected when only nylon-wool adherent cells were incubated with DAF. Thus, DAF exerted its effect through binding to acceptor cells which were included in nylon-wool nonadherent spleen cells from normal mice.
我们发现，在用异种红细胞致敏的小鼠血清中存在一种能够增强迟发型超敏反应（DTH）以诱导足垫迟发反应（DFR）的抗原特异性因子，或者在致敏T细胞与特异性抗原混合物的培养上清液中也存在该因子。这种因子（迟发型超敏反应增强因子；DAF）在移植受体中被证实可增强迟发型超敏反应。在本文中，利用迟发型超敏反应的体外诱导和局部移植系统对DAF的这种活性进行了进一步研究。当用12小时前注射含DAF血清的小鼠脾细胞或在冰上与含DAF血清孵育2小时的脾细胞与异种红细胞一起培养时，DAF也增强了迟发型超敏反应的初次体外诱导。DAF作用于迟发型超敏反应的诱导阶段，并增强了依赖于Thy-1阳性T细胞的典型迟发型超敏反应。DAF具有抗原特异性，但不属于传统免疫球蛋白。当尼龙毛非黏附细胞在培养这些细胞和抗原之前与DAF孵育时可检测到DAF的活性，但仅尼龙毛黏附细胞与DAF孵育时则检测不到该活性。因此，DAF通过与正常小鼠尼龙毛非黏附脾细胞中所含的受体细胞结合发挥其作用。
