Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Brazil.
Department of Chemistry, State University of Maringá, Maringá, Brazil.
Front Cell Infect Microbiol. 2019 Jun 26;9:230. doi: 10.3389/fcimb.2019.00230. eCollection 2019.
is the major pathogen isolated from nosocomial bloodstream infections, leading to higher mortality rates. Thus, due to its clinical relevance, studies aiming to understand host-pathogen interactions in infection are necessary. Therefore, we performed proteomic analysis using a murine model of serial systemic infection by to evaluate possible changes in the protein profile of the pathogen over time. Firstly, we observed a reduction in the median survival time of infected animals with increasing passage number, suggesting a higher pathogenicity acquired during repeated infections. By LC-MS/MS, it was possible to obtain protein profiles from the wild-type strain (WT) and compare them to proteins extracted from cells recovered from infected tissues during passages one, three, and four (P1, P3, and P4). We obtained 56, 29, and 97 proteins in P1, P3, P4, respectively, all varying in abundance. Regarding biological processes, the majority of proteins were related to carbohydrate metabolism, stress responses and amino acid metabolism. The proteins were also categorized according to their potential role in virulence traits, such as biofilm production, yeast-to-hyphae transition, phenotypic switching, proteins related to stress responses, and uncharacterized proteins. Therefore, serial infection in combination with proteomic approach enabled us to deepen the existing knowledge about host-pathogen interactions.
是医院血流感染的主要病原体，导致更高的死亡率。因此，由于其临床相关性，有必要研究旨在了解 感染中的宿主-病原体相互作用的研究。因此，我们使用连续系统感染的小鼠模型进行蛋白质组学分析，以评估病原体蛋白谱随时间的可能变化。首先，我们观察到随着传代次数的增加，感染动物的中位生存时间缩短，这表明在反复感染过程中获得了更高的致病性。通过 LC-MS/MS，我们从野生型（WT）菌株中获得了蛋白质图谱，并将其与从感染组织中回收的第 1、3 和 4 代（P1、P3 和 P4）的 细胞中提取的蛋白质进行了比较。我们分别在 P1、P3 和 P4 中获得了 56、29 和 97 种蛋白质，其丰度均有所变化。关于生物学过程，大多数蛋白质与碳水化合物代谢、应激反应和氨基酸代谢有关。根据它们在毒力特性中的潜在作用，如生物膜形成、酵母-菌丝过渡、表型转换、与应激反应相关的蛋白质和未鉴定的蛋白质，对蛋白质进行了分类。因此，连续感染与蛋白质组学方法相结合，使我们能够加深对宿主-病原体相互作用的现有认识。
