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来自罗勒属植物的具有促进植物生长能力的内生细菌

Endophytic bacteria with plant growth promoting abilities from L.

作者信息

Mukherjee Ananya, Bhattacharjee Puja, Das Rituparna, Pal Arundhati, Paul Amal K

机构信息

Microbiology Laboratory, Department of Botany, University of Calcutta, Kolkata, India.

Department of Botany, Serampore College, Serampore, Hooghly, India.

出版信息

AIMS Microbiol. 2017 Jul 14;3(3):596-612. doi: 10.3934/microbiol.2017.3.596. eCollection 2017.

Abstract

Endophytic bacteria colonizing the internal tissues of plants are known to improve plant growth by a wide variety of mechanisms. This study envisages the isolation and evaluation of plant growth promoting attributes of bacterial endophytes in perennial fern L. A total of 20 phenotypically distinguishable bacterial endophytes were isolated from surface sterilized leaf lamina, petiole, rhizome and spike of L. The Shannon-Weaver diversity index showed that the rhizome (1.54) harbor more diverse types of endophytic bacteria than in its petiole, leaf lamina and spike. The isolated endophytes were characterized on the basis of micromorphological and physio-biochemical characters and tentatively assigned to the genus , and . The isolates showed distinct variations in their enzymatic activities, sugar fermentation and antibiotic sensitivity profile. A number of endophytic isolates showed plant growth promoting activities like production of indole-3-acetic acid (IAA) and siderophore, growth in nitrogen-free medium and solubilization of phosphate. Time course of growth and IAA production by the potent isolate OPR 7 have been determined. Exploitation of such plant growth promoting endophytes appears to be one of the best options in increasing biomass yield and improving plant fitness and productivity.

摘要

已知定殖于植物内部组织的内生细菌可通过多种机制促进植物生长。本研究旨在从多年生蕨类植物L.中分离并评估内生细菌促进植物生长的特性。从表面消毒的L.叶片、叶柄、根茎和穗中总共分离出20种表型可区分的内生细菌。香农 - 韦弗多样性指数表明,根茎(1.54)中内生细菌的种类比叶柄、叶片和穗中的更多样。根据微观形态和生理生化特征对分离出的内生菌进行了鉴定,并初步归为属、属和属。这些分离株在酶活性、糖发酵和抗生素敏感性方面表现出明显差异。许多内生分离株表现出促进植物生长的活性,如吲哚 - 3 - 乙酸(IAA)和铁载体的产生、在无氮培养基中的生长以及磷酸盐的溶解。已测定了强效分离株OPR 7的生长和IAA产生的时间进程。利用此类促进植物生长的内生菌似乎是提高生物量产量、改善植物适应性和生产力的最佳选择之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3788/6604999/a665ce9efcd4/microbiol-03-03-596-g001.jpg

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