石榴蚜及其捕食者和保护者内生细菌的次生生物活性化合物的 GC 分析、抗癌和抗菌活性。
GC Analysis, Anticancer, and Antibacterial Activities of Secondary Bioactive Compounds from Endosymbiotic Bacteria of Pomegranate Aphid and Its Predator and Protector.
机构信息
Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
High Altitude Research Centre, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
出版信息
Molecules. 2023 May 22;28(10):4255. doi: 10.3390/molecules28104255.
Bacterial secondary metabolites are a valuable source of various molecules that have antibacterial and anticancer activity. In this study, ten endosymbiotic bacteria of aphids, aphid predators and ants were isolated. Bacterial strains were identified according to the 16S rRNA gene. Ethyl acetate fractions of methanol extract (EA-ME) were prepared from each isolated bacterium and tested for their antibacterial activities using the disk diffusion method. The EA-ME of three bacterial species, sp., , , from the pomegranate aphids , , and , respectively, exhibited elevated antibacterial activity against one or several of the five pathogenic bacteria tested. The inhibition zones ranged from 10.00 ± 0.13 to 20.00 ± 1.11 mm, with minimum inhibitory concentration (MIC) values ranging from 0.156 mg/mL to 1.25 mg/mL. The most notable antibacterial activity was found in the EA-ME of against and , with an MIC value of 0.156 mg/mL. The cytotoxic activity of EA-ME was dependent on the cell line tested. The most significant cytotoxicity effect was observed for extracts of and , at 12.5 µg/mL, against the epithelial cells of lung carcinoma (A549), with a cell reduction of 79.4% and 67.2%, respectively. For the EA-ME of and at 12.5 µg/mL, 69.4% and 67.8% cell reduction were observed against human colon cancer (Hct116), respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of three EA-ME revealed the presence of several bioactive secondary metabolites that have been reported previously to possess antibacterial and anticancer properties. To the best of our knowledge, this is the first study to examine the biological activities of endosymbiotic bacteria in aphids, aphid predators and ants. The promising data presented in this study may pave the way for alternative drugs to overcome the continued emergence of multidrug-resistant bacteria, and find alternative drugs to conventional cancer therapies.
细菌次生代谢产物是具有抗菌和抗癌活性的各种分子的宝贵来源。在这项研究中，从蚜虫、蚜虫捕食者和蚂蚁的内共生细菌中分离出了 10 株。根据 16S rRNA 基因鉴定细菌菌株。从每个分离的细菌中制备甲醇提取物（EA-ME）的乙酸乙酯部分，并使用圆盘扩散法测试其抗菌活性。三种细菌物种的 EA-ME， ， ，分别来自石榴蚜虫 ， ，和 ，对测试的五种致病菌中的一种或几种表现出较高的抗菌活性。抑菌圈直径为 10.00 ± 0.13 至 20.00 ± 1.11 毫米，最小抑菌浓度（MIC）值为 0.156 毫克/毫升至 1.25 毫克/毫升。对 和 的 EA-ME 表现出最显著的抗菌活性，MIC 值为 0.156 毫克/毫升。EA-ME 的细胞毒性活性取决于所测试的细胞系。在 12.5 µg/mL 时，对肺腺癌（A549）上皮细胞的抑制作用最为显著，提取物 和 的抑制率分别为 79.4%和 67.2%。对于 和 的 EA-ME，在 12.5 µg/mL 时，对人结肠癌细胞（Hct116）的细胞减少率分别为 69.4%和 67.8%。对三种 EA-ME 的气相色谱-质谱（GC-MS）分析表明，存在几种以前报道具有抗菌和抗癌特性的生物活性次生代谢产物。据我们所知，这是首次研究蚜虫、蚜虫捕食者和蚂蚁内共生细菌的生物学活性。本研究中提出的有希望的数据可能为克服持续出现的多药耐药菌提供替代药物铺平道路，并找到替代传统癌症治疗方法的药物。
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