State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , China.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering , Qingdao University of Science and Technology , Qingdao 266042 , China.
ACS Appl Mater Interfaces. 2019 Jul 24;11(29):25702-25707. doi: 10.1021/acsami.9b07523. Epub 2019 Jul 11.
This work reports the innovative design and application of a three-dimensional (3D) TiO@CuO@nickel foam electrode synergized with enzyme catalysis toward the proof-of-concept study for oxygen-independent photocathodic enzymatic detection. Specifically, a 3D-nanostructured photoelectrode has great potential in the semiconductor-based photoelectrochemical (PEC) biological analysis. On the other hand, using various photocathodes, cathodic PEC bioanalysis, especially the photocathodic enzymatic detection, represents an attractive frontier in the field. Different from state-of-the-art photocathodic enzymatic studies that are oxygen-dependent, herein, we present the ingenious design, characterization, and implementation of 3D TiO@CuO@nickel foam photocathodes for the first oxygen-independent example. In such a configuration, the CuO acted as the visible-light absorber, while the TiO shell would simultaneously function as a protective layer for CuO and as a desirable substrate for the immobilization of enzyme biomolecules. Especially, because of the proper band positions, the as-designed photocathode exhibited unique O-independent PEC property. Exemplified by glucose oxidases, the as-developed sensor exhibited positive response to glucose with good performance. Because various oxidases could be integrated with the system, this protocol could serve as a universal O-independent platform for many other targets. This work is also anticipated to catalyze more studies in the advanced 3D photoelectrodes toward innovative enzymatic applications.
这项工作报道了一种三维（3D）TiO@CuO@泡沫镍电极的创新设计和应用，该电极协同酶催化，用于氧非依赖性光阴极酶检测的概念验证研究。具体来说，3D 纳米结构光电极在基于半导体的光电化学（PEC）生物分析中具有很大的潜力。另一方面，使用各种光电阴极，阴极 PEC 生物分析，特别是光阴极酶检测，代表了该领域一个有吸引力的前沿。与依赖氧气的最新光阴极酶研究不同，本文首次提出了用于氧非依赖性的 3D TiO@CuO@泡沫镍光电阴极的巧妙设计、表征和实施。在这种配置中，CuO 充当可见光吸收剂，而 TiO 壳同时充当 CuO 的保护层和酶生物分子固定的理想基底。特别是，由于适当的能带位置，所设计的光电阴极表现出独特的氧非依赖性 PEC 特性。以葡萄糖氧化酶为例，所开发的传感器对葡萄糖表现出良好的响应性能。由于可以将各种氧化酶与该系统集成，因此该方案可以作为许多其他目标的通用氧非依赖性平台。这项工作也有望促进更多关于先进 3D 光电极的研究，以实现创新的酶应用。
