Laboratory of Environmental Toxicology, Department of Sanitary and Environmental Engineering, Federal University of Santa Catarina, Florianópolis, SC 88040-970, Brazil.
Laboratory of Plant Cell Biology, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88049-900, Brazil.
Toxicol In Vitro. 2019 Dec;61:104596. doi: 10.1016/j.tiv.2019.104596. Epub 2019 Jul 8.
The role of the crystalline structure on the toxicity of two phases of AlO NPs, alpha (α-AlO NPs) and eta (η-AlO NPs), was investigated in this study. Different techniques were employed for the characterization of the AlO NPs and multiple toxicological endpoints were used to assess the toxicity toward mouse neuroblastoma (N2A) and human bronchial epithelial (BEAS-2B) cells. Based on the results of the multiple toxicological endpoints, revealed differences in the toxic potential results for α-AlO NPs and η-AlO NPs, with the latter showing a more pronounced effect. These effects could be due to the high uptake of the η-AlO NPs in the cytoplasmic vesicles, as evidenced by TEM and ICP-MS. Hence, the results demonstrate the potential toxicity of both α-AlO NPs and η-AlO NPs, although the N2A and BEAS-2B cells showed greater susceptibility toward η-AlO NPs. Thus, our study demonstrates the important role of the crystalline structure in relation to the nanotoxicity of AlO NPs.
本研究考察了两种形态的 AlO NPs（α-AlO NPs 和 η-AlO NPs）的晶体结构对其毒性的作用。采用多种技术对 AlO NPs 进行了表征，并使用多种毒理学终点来评估其对小鼠神经母细胞瘤（N2A）和人支气管上皮（BEAS-2B）细胞的毒性。基于多种毒理学终点的结果，α-AlO NPs 和 η-AlO NPs 的毒性潜力结果存在差异，后者表现出更明显的作用。这些影响可能是由于 η-AlO NPs 被大量内吞到细胞质小泡中，这一点通过 TEM 和 ICP-MS 得到了证实。因此，研究结果表明，两种 AlO NPs 都具有潜在的毒性，尽管 N2A 和 BEAS-2B 细胞对 η-AlO NPs 的敏感性更高。因此，本研究表明，晶体结构在 AlO NPs 的纳米毒性方面起着重要作用。
