Department of Cell Ultrastructure, Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033 Irkutsk, Russia.
Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan.
Int J Mol Sci. 2023 Oct 13;24(20):15146. doi: 10.3390/ijms242015146.
The effect is studied of water-suspended soot microparticles on the actin cytoskeleton, apoptosis, and proliferation in the gill epithelium of pearl gourami. To this end, the fish are kept in aquariums with 0.005 g/L of soot for 5 and 14 days. Laser confocal microscopy is used to find that at the analyzed times of exposure to the pollutant zones appear in the gill epithelium, where the actin framework of adhesion belts dissociates and F-actin either forms clumps or concentrates perinuclearly. It is shown that the exposure to soot microparticles enhances apoptosis. On day 5, suppression of the proliferation of cells occurs, but the proliferation increases to the control values on day 14. Such a paradoxical increase in proliferation may be a compensatory process, maintaining the necessary level of gill function under the exposure to toxic soot. This process may occur until the gills' recovery reserve is exhausted. In general, soot microparticles cause profound changes in the actin cytoskeleton in gill cells, greatly enhance cell death, and influence cell proliferation as described. Together, these processes may cause gill dysfunction and affect the viability of fish.
研究了水悬浮烟尘微粒对珍珠鱼鳃上皮细胞中的肌动蛋白细胞骨架、细胞凋亡和增殖的影响。为此，将鱼饲养在装有 0.005 克/升烟尘的水族箱中 5 天和 14 天。激光共聚焦显微镜发现，在分析暴露于污染物的时间时，鳃上皮中出现了区域，其中附着带的肌动蛋白框架解离，F-肌动蛋白要么形成团块，要么集中在核周。结果表明，烟尘微粒的暴露会增强细胞凋亡。第 5 天，细胞增殖受到抑制，但第 14 天增殖增加到对照值。这种增殖的矛盾增加可能是一种补偿过程，在暴露于有毒烟尘的情况下维持必要的鳃功能水平。这个过程可能会持续到鳃的恢复储备耗尽。总的来说，烟尘微粒会导致鳃细胞中肌动蛋白细胞骨架发生深刻变化，大大增强细胞死亡，并如所述影响细胞增殖。这些过程可能会导致鳃功能障碍并影响鱼类的生存能力。
