Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.
State Key Laboratory of Bioreactor Engineering, School of Resource and Environmental, East China University of Science and Technology, Shanghai 200237, China.
Infect Genet Evol. 2019 Nov;75:103954. doi: 10.1016/j.meegid.2019.103954. Epub 2019 Jul 8.
Few studies have been conducted on the distribution of Cryptosporidium species and subtypes in equine animals. In this study, 878 stool specimens were collected during 2015-2019 from 551 donkeys and 327 horses in Shandong, Xinjiang, and Inner Mongolia, China and screened for Cryptosporidium spp. by PCR analysis of the small subunit rRNA gene. The Cryptosporidium species presented were identified by sequence analysis of the PCR products and subtyped by sequence analysis of the 60 kDa glycoprotein gene. The infection rates of Cryptosporidium spp. in horses and donkeys were 3.1% (10/327) and 14.5% (80/551), respectively. Four Cryptosporidium species/genotypes were identified, including C. parvum (in 5 horses), C. hominis (in 75 donkeys), Cryptosporidium horse genotype (in 5 horses and 4 donkeys) and a new genotype that is genetically related to Cryptosporidium mink genotype (in 1 donkey). All C. parvum isolates were subtyped as IIdA19G1, C. hominis as IkA16G1, and horse genotype as VIaA15G4. Data from this study indicate that four Cryptosporidium species are circulating in horses and donkeys in the study areas, with C. hominis as a dominant Cryptosporidium species in only donkeys. Attention should be paid to reduce the transmission of these zoonotic Cryptosporidium spp.
鲜有研究针对马属动物中的隐孢子虫种及亚型分布展开。本研究于 2015 年至 2019 年，采集了来自中国山东、新疆和内蒙古的 551 头驴和 327 匹马的 878 份粪便标本，通过 PCR 分析检测粪便中小亚基 rRNA 基因以筛查隐孢子虫属。通过对 PCR 产物的序列分析鉴定隐孢子虫种，通过对 60kDa 糖蛋白基因的序列分析进行亚型鉴定。马属动物的隐孢子虫属感染率分别为 3.1%（10/327）和 14.5%（80/551）。共鉴定出 4 种隐孢子虫/基因型，包括 C. parvum（5 匹马）、C. hominis（75 头驴）、马源隐孢子虫基因型（5 匹马和 4 头驴）和一种与水貂源隐孢子虫基因型亲缘关系较近的新基因型（1 头驴）。所有 C. parvum 分离株均被亚分型为 IIdA19G1，C. hominis 为 IkA16G1，马源基因型为 VIaA15G4。本研究数据表明，研究地区的马和驴中存在 4 种隐孢子虫，且仅在驴中 C. hominis 为优势隐孢子虫种。应注意减少这些人畜共患隐孢子虫属的传播。
