沙特阿拉伯新序列型 ST1688 临床鲍曼不动杆菌分离株的基因组草图
Draft genome sequence of a clinical Acinetobacter baumannii isolate of new sequence type ST1688 from Saudi Arabia.
机构信息
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
出版信息
J Glob Antimicrob Resist. 2019 Sep;18:151-152. doi: 10.1016/j.jgar.2019.06.021. Epub 2019 Jul 8.
OBJECTIVES
Acinetobacter baumannii has emerged as a prevalent multidrug-resistant nosocomial pathogen worldwide. Here we report the draft genome sequence of A. baumannii strain Ab174 isolated from a neonatal patient diagnosed with acute peritonitis.
METHODS
The draft genome sequence of A. baumannii Ab174 was determined using a MiSeq platform (Illumina Inc., San Diego, CA) using v.3, 2×30-bp chemistry. Genomic assembly was performed using SPAdes 3.9 algorithm.
RESULTS
The draft genome of A. baumannii Ab174 is 3 747 065bp in length and was classified as a new sequence type (ST1688). The genome of A. baumannii Ab174 has a G+C content of 39% and harbours two plasmids. The antimicrobial resistance gene bla and the virulence factor gene for penicillin-binding protein G (pbpG) as well as 17 genomic islands and 14 insertion sequences were identified in the genome of A. baumannii Ab174.
CONCLUSION
The genome sequence of A. baumannii strain Ab174 can be used as a reference sequence for the new ST1688. These data will facilitate further understanding of genomic variation in isolates from different geographical regions.
目的
鲍曼不动杆菌已成为全球流行的多药耐药医院病原体。本研究报告了从诊断为急性腹膜炎的新生儿患者中分离的鲍曼不动杆菌 Ab174 菌株的基因组草图序列。
方法
使用 MiSeq 平台（Illumina Inc.，圣地亚哥，CA），采用 v.3、2×30-bp 化学法测定 Ab174 鲍曼不动杆菌的基因组草图序列。使用 SPAdes 3.9 算法进行基因组组装。
结果
Ab174 鲍曼不动杆菌的基因组草图长 3747065bp，被归类为新的序列型（ST1688）。Ab174 鲍曼不动杆菌基因组的 G+C 含量为 39%，并包含两个质粒。在 Ab174 鲍曼不动杆菌的基因组中鉴定出了抗菌药物耐药基因 bla 和青霉素结合蛋白 G（pbpG）的毒力因子基因，以及 17 个基因组岛和 14 个插入序列。
结论
Ab174 鲍曼不动杆菌株的基因组序列可用作新 ST1688 的参考序列。这些数据将有助于进一步了解来自不同地理区域的分离株的基因组变异。
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