Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Department of General Practice, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310018, China.
J Glob Antimicrob Resist. 2019 Dec;19:43-45. doi: 10.1016/j.jgar.2019.08.015. Epub 2019 Aug 23.
The prevalence of multidrug-resistant Acinetobacter baumannii is of serious concern in hospital settings. Here we report the genome sequence and genomic characterisation of a clinical A. baumannii isolate from China belonging to a novel sequence type (ST) harbouring bla and a new ampC allelic variant bla simultaneously.
Whole genomic DNA from A. baumannii A42 was extracted and sequenced using an Illumina HiSeq X10 platform. De novo genome assembly was performed using Unicycler, and the draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline. Genomic analyses were performed through various bioinformatics web servers from the Center for Genomic Epidemiology as well as BacWGSTdb.
The genome size was calculated as 3 800 237bp, with 3610 protein-coding sequences and a GC content of 38.9%. A. baumannii A42 belongs to a rare sporadic clone ST1928. The resistome contains genes encoding resistance to β-lactams (bla and a new ampC allelic variant bla). Virulence factor genes encoding biofilm-associated protein (bap), acinetobactin biosynthesis protein (basA-J), penicillin-binding protein (pbpG) and biofilm synthesis N-glycosyltransferase (pgaA-D) as well as 16 genomic islands and multiple insertion sequences were also identified in the genome of A. baumannii A42.
This is the first report of the genome sequence of an A. baumannii ST1928 clinical isolate carrying a novel class C β-lactamase gene from China. The genome sequence data can be used as a reference sequence for comparative studies and would facilitate further understanding of the antimicrobial resistance mechanisms of A. baumannii at the genomic level.
鲍曼不动杆菌的多药耐药性令人严重关切，尤其是在医院环境中。在此，我们报告了一株来自中国的临床分离的鲍曼不动杆菌，其属于一个新型序列型（ST），同时携带 bla 和一种新的 AmpC 等位基因 bla。
采用 Illumina HiSeq X10 平台提取并测序鲍曼不动杆菌 A42 的全基因组 DNA。使用 Unicycler 进行从头基因组组装，使用 NCBI Prokaryotic Genome Annotation Pipeline 对草案基因组进行注释。通过来自基因组流行病学中心和 BacWGSTdb 的各种生物信息学网络服务器进行基因组分析。
基因组大小计算为 3800237bp，包含 3610 个蛋白质编码序列，GC 含量为 38.9%。A. baumannii A42 属于罕见的散发性克隆 ST1928。耐药基因组包含编码对β-内酰胺类药物（bla 和一种新的 AmpC 等位基因 bla）耐药的基因。毒力因子基因编码生物膜相关蛋白（bap）、不动杆菌素生物合成蛋白（basA-J）、青霉素结合蛋白（pbpG）和生物膜合成 N-糖基转移酶（pgaA-D），以及 16 个基因组岛和多个插入序列，也在 A. baumannii A42 的基因组中被鉴定出来。
这是首次报道来自中国的携带新型 C 类β-内酰胺酶基因的 ST1928 临床分离株的鲍曼不动杆菌基因组序列。基因组序列数据可用作比较研究的参考序列，并有助于进一步了解鲍曼不动杆菌在基因组水平上的抗菌药物耐药机制。
