The Ruth and Bruce Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa 31096, Israel.
Research Unit at Geha Mental Health Center and Laboratory of Biological Psychiatry at Felsenstein Medical Research Center, Petah Tikva 4910002, Israel.
Cells. 2019 Jul 10;8(7):694. doi: 10.3390/cells8070694.
The 18 kDa translocator protein (TSPO) is an initiator of the mitochondrial apoptosis cascade. Cigarette smoke (CS) exposure provokes alterations in TSPO expression as well as upregulation of its related functions such as mitochondrial membrane potential (Δψ) and reactive oxygen species generation, which are associated with cell death. In the current study, H1299 lung cancer cell line exposed to CS for various time periods (30 mins, 60 mins and 120 mins) and TSPO expression and cell death processes were studied. CS exposure for 30 mins resulted in a non-significant increase in TSPO expression by 24% ( > 0.05 vs. control). CS exposure for 60 mins and 120 mins resulted in a significant increase by 43% ( < 0.05 vs. control) and by 47% ( < 0.01 vs. control), respectively. Furthermore, TSPO-related mitochondrial functions were upregulated at the 120 mins time point following CS exposure. TSPO expression is upregulated by CS, suggesting that TSPO plays a role in cell death processes induced by CS exposure. Alterations in TSPO-related cell death processes suggest that TSPO may be involved in the tissue damage caused by CS.
18kDa 转位蛋白（TSPO）是线粒体凋亡级联反应的启动子。香烟烟雾（CS）暴露会引起 TSPO 表达的改变，以及其相关功能的上调，如线粒体膜电位（Δψ）和活性氧生成，这与细胞死亡有关。在本研究中，研究了 H1299 肺癌细胞系暴露于 CS 不同时间段（30 分钟、60 分钟和 120 分钟）后 TSPO 表达和细胞死亡过程。CS 暴露 30 分钟导致 TSPO 表达增加了 24%（>0.05 与对照相比），但无统计学意义。CS 暴露 60 分钟和 120 分钟分别导致 TSPO 表达增加了 43%（<0.05 与对照相比）和 47%（<0.01 与对照相比）。此外，CS 暴露 120 分钟后，TSPO 相关的线粒体功能也被上调。CS 上调 TSPO 的表达，提示 TSPO 在 CS 暴露诱导的细胞死亡过程中起作用。TSPO 相关细胞死亡过程的改变提示 TSPO 可能参与 CS 引起的组织损伤。
