Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2019 Jul 10;20(14):3396. doi: 10.3390/ijms20143396.
The leaf (OHPL) is a new bioactive resource with potential antidepressant activity, but few reports have confirmed its chemical composition or antidepressant effect. To investigate the phytochemical profile of OHPL ethanol extract (OHPLE), six flavone -glycosides and two flavone -glycosides were purified by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography (HSCCC-prep-HPLC). The eight isolated compounds were identified by NMR and MS. Forty-six flavonoids, including flavones, flavone -glycosides, flavone -glycosides, isoflavones, isoflavone -glycosides, prenylflavones and polymethoxyflavones were definitively or tentatively identified from OHPLE using ultra-performance liquid chromatography/ electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS/MS) on the basis of fragment ions that are characteristic of these isolated compounds. The results of the antidepressant assay suggest that OHPLE significantly improved depression-related behaviors of chronic unpredictable mild stress (CUMS) mice. The observed changes in these mice after OHPLE treatment were an increased sucrose preference index, reduced feeding latency, prolonged tail suspension time, and upregulated expression of brain-derived neurotrophic factor (BDNF). The details of the phytochemicals and the antidepressant effect of OHPLE are reported here for the first time. This study indicates that the OHPL, enriched in flavone -glycosides, is a new resource that might be potentially applied in the field of nutraceuticals (or functional additives) with depression-regulating functions.
该叶片（OHPL）是具有潜在抗抑郁活性的新生物活性资源，但很少有报道证实其化学成分或抗抑郁作用。为了研究 OHPL 乙醇提取物（OHPLE）的植物化学成分，通过高速逆流色谱结合制备高效液相色谱（HSCCC-Prep-HPLC）纯化了六种黄酮苷和两种黄酮苷。通过 NMR 和 MS 鉴定了八种分离化合物。使用超高效液相色谱/电喷雾电离四极杆飞行时间质谱（UPLC-ESI-QTOF-MS/MS），从 OHPLE 中鉴定出 46 种类黄酮，包括黄酮、黄酮苷、黄酮苷、异黄酮、异黄酮苷、prenylflavones 和多甲氧基黄酮，基于这些分离化合物的特征碎片离子。抗抑郁测定的结果表明，OHPLE 显著改善了慢性不可预测轻度应激（CUMS）小鼠的抑郁相关行为。OHPLE 处理后这些小鼠的观察到的变化包括蔗糖偏好指数增加、摄食潜伏期减少、悬尾时间延长以及脑源性神经营养因子（BDNF）表达上调。首次报道了 OHPL 的植物化学成分和抗抑郁作用的详细信息。本研究表明，富含黄酮苷的 OHPL 是一种新的资源，可能在具有调节抑郁功能的营养保健品（或功能性添加剂）领域得到潜在应用。
