Kwon Kyoung Wan, Nam Yoonjin, Choi Won Seok, Kim Tae Wook, Kim Geon Min, Sohn Uy Dong
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea.
Korean J Physiol Pharmacol. 2019 Jul;23(4):263-270. doi: 10.4196/kjpp.2019.23.4.263. Epub 2019 Jun 25.
Hydrogen sulfide is well-known to exhibit anti-inflammatory and cytoprotective activities, and also has protective effects in the liver. This study aimed to examine the protective effect of hydrogen sulfide in rats with hepatic encephalopathy, which was induced by mild bile duct ligation. In this rat model, bile ducts were mildly ligated for 26 days. Rats were treated for the final 5 days with sodium hydrosulfide (NaHS). NaHS (25 µmol/kg), 0.5% sodium carboxymethyl cellulose, or silymarin (100 mg/kg) was administered intraperitoneally once per day for 5 consecutive days. Mild bile duct ligation caused hepatotoxicity and inflammation in rats. Intraperitoneal NaHS administration reduced levels of aspartate aminotransferase and alanine aminotransferase, which are indicators of liver disease, compared to levels in the control mild bile duct ligation group. Levels of ammonia, a major causative factor of hepatic encephalopathy, were also significantly decreased. Malondialdehyde, myeloperoxidase, catalase, and tumor necrosis factor-α levels were measured to confirm antioxidative and anti-inflammatory effects. N-Methyl-D-aspartic acid (NMDA) receptors with neurotoxic activity were assessed for subunit NMDA receptor subtype 2B. Based on these data, NaHS is suggested to exhibit hepatoprotective effects and guard against neurotoxicity through antioxidant and anti-inflammatory actions.
众所周知，硫化氢具有抗炎和细胞保护活性，对肝脏也有保护作用。本研究旨在探讨硫化氢对轻度胆管结扎诱导的大鼠肝性脑病的保护作用。在该大鼠模型中，胆管轻度结扎26天。大鼠在最后5天用硫氢化钠（NaHS）治疗。连续5天每天腹腔注射一次NaHS（25 µmol/kg）、0.5%羧甲基纤维素钠或水飞蓟宾（100 mg/kg）。轻度胆管结扎导致大鼠肝毒性和炎症。与对照轻度胆管结扎组相比，腹腔注射NaHS降低了作为肝病指标的天冬氨酸转氨酶和丙氨酸转氨酶水平。肝性脑病的主要致病因素氨水平也显著降低。测量丙二醛、髓过氧化物酶、过氧化氢酶和肿瘤坏死因子-α水平以确认抗氧化和抗炎作用。评估具有神经毒性活性的N-甲基-D-天冬氨酸（NMDA）受体的亚基NMDA受体亚型2B。基于这些数据，提示NaHS通过抗氧化和抗炎作用发挥肝脏保护作用并预防神经毒性。
